Indigenous marine diatoms as novel sources of bioactive peptides with antihypertensive and antioxidant properties. (4th November 2018)

• Record Type: Journal Article
• Authors: Barkia, Ines; Al‐Haj, Lamya; Abdul Hamid, Azizah; Zakaria, Mutaharah; Saari, Nazamid; Zadjali, Fahad
• Is Part Of: International journal of food science & technology. Volume 54:Number 5(2019)
• Page Start: 1514
• Page End: 1522
• Publication Date: 2018-11-04
• Subjects: ACE‐inhibitory activity -- antihypertensive activity -- antioxidant activity -- marine microalgae -- protein hydrolysates; Food industry and trade -- Periodicals; 664
• DOI: 10.1111/ijfs.14006
• Languages: English
• ISSNs: 0950-5423

Abstract

Summary: Several bioactive compounds from microalgae have demonstrated diverse biological activities with positive effects on human health. However, the potential of bioactive peptides as functional foods is still undervalued. Therefore, the exploration of microalgae strains as sources of bioactive peptides could reveal strong and unique bioactivities, especially when these marine sources have never been explored before. For this aim, protein extracts from six indigenous marine diatoms were subjected to enzymatic hydrolysis using four proteases (flavourzyme, pepsin, papain and trypsin). The hydrolysates were then tested for angiotensin converting enzyme (ACE)‐inhibitory, antioxidant and antihypertensive properties. Results showed that papain hydrolysates from all microalgae strains exhibited strong ACE‐inhibitory activities and antioxidant properties. In particular, protein hydrolysates from Bellerochea malleus were found to reduce blood pressure properties of 17 mmHg after 5 days of oral administration to SHR animals. These results revealed the potential of bioactive peptides from indigenous marine diatoms for use as functional foods or nutraceuticals. Abstract : The dried microalgal biomass from six microalgae isolates was subjected to protein extraction using Three Phase Partitioning (TPP). Then, protein extracts were hydrolysed using commercial proteases (flavourzyme, pepsin, papain and trypsin). Afterwards, the resultant protein hydrolysates were assessed for their ACE‐inhibitory activity and antioxidant properties in vitro as well as antihypertensive effects in animal models (SHR).