Proteomic and physiological assessment of stress sensitive and tolerant variety of tomato treated with brassinosteroids and hydrogen peroxide under low-temperature stress. (15th August 2019)

• Record Type: Journal Article
• Authors: Khan, Tanveer Alam; Yusuf, Mohammad; Ahmad, Aqeel; Bashir, Zoobia; Saeed, Taiba; Fariduddin, Qazi; Hayat, Shamsul; Mock, Hans-Peter; Wu, Tingquan
• Is Part Of: Food chemistry. Volume 289(2019)
• Page Start: 500
• Page End: 511
• Publication Date: 2019-08-15
• Subjects: Brassinosteroids -- Hydrogen peroxide -- Low temperature -- Lycopersicon esculentum -- Proteomics -- 2-DE; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.03.029
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Low temperature declined plant growth, and metabolism parameters in tomato. The combination of EBL and H2 O2 enhanced antioxidants and proline. Low-temperature diminished expression of 20 proteins in EBL + H2 O2 treated plants. Overall, O80577, Q9FJQ8, and Q9SKL2 took part in the citrate cycle pathway. Abstract: The aim of current investigation was to perform proteomics and physio-chemical studies to dissect the changes in contrasting varieties (S-22 and PKM-1) of Lycopersicon esculentum under low-temperature stress. Plant grown under variable low-temperature stress were analysed for their growth biomarkers, antioxidant enzyme activities, and other physiological parameters, which headed toward the determination of protein species responding to low-temperature and 24-epibrassinolide (EBL) concentrations. The plants grown under temperatures, 20/14, 12/7, and 10/3 °C recorded significantly lower growth biomarkers, SPAD chlorophyll, net photosynthetic rate and carbonic anhydrase activity in S-22 and PKM-1. Moreover, the combined effect of EBL and hydrogen peroxide (H2 O2 ) significantly improved the parameters mentioned above and consecutively upgraded the different antioxidant enzymes (CAT and SOD) with higher accumulation of proline under stress and stress-free environments. Furthermore, proteomics study revealed that the maximum number of differentially expressed proteins were detected in S-22 (EBL + H2 O2 ); while treatment with EBL + H2 O2 + low temperature lost expression of 20 proteins. Overall, three proteins (O80577, Q9FJQ8, and Q9SKL2) took a substantial part in the biosynthesis of citrate cycle pathway and enhanced the growth and photosynthetic efficiency of tomato plants under low-temperature stress.