Immunomodulatory effects of an acidic polysaccharide fraction from herbal Gynostemma pentaphyllum tea in RAW264.7 cells. Issue 4 (3rd April 2019)

• Record Type: Journal Article
• Authors: Ren, Daoyuan; Zhao, Yan; Zheng, Quan; Alim, Aamina; Yang, Xingbin
• Is Part Of: Food & function. Volume 10:Issue 4(2019)
• Page Start: 2186
• Page End: 2197
• Publication Date: 2019-04-03
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c9fo00219g
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : A new acidic polysaccharide (GPTP-3) with a molecular weight of 2.49 × 10 6 Da was extracted and purified from Gynostemma pentaphyllum tea. Abstract : A new acidic polysaccharide (GPTP-3) with a molecular weight of 2.49 × 10 6 Da was extracted and purified from Gynostemma pentaphyllum tea. Monosaccharide analysis revealed that GPTP-3 mainly comprised mannose (20.4%), glucuronic acid (17.4%), glucose (33.4%), and galactose (21.4%) (parentheses indicate the molar percentages). Immunostimulating assays indicated that GPTP-3 could markedly promote the secretion of NO, TNF-α, IL-1β, and IL-6 in murine macrophage RAW264.7. TLR4 was found to be a recognized target of GPTP-3. Moreover, TLR4-related mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt, including ERK, JNK, p38, and Akt, were rapidly activated by GPTP-3 in RAW264.7 cells. Furthermore, GPTP-3 was found to induce the nuclear translocation of NF-κB subunit p65. All these findings suggest that MAPK, PI3K/Akt, and NF-κB pathways are involved in GPTP-3-induced macrophage activations, and GPTP-3 has the potential to be developed as a functional food with immunomodulatory functions.