Essential oils of Moldavian Thymus species: Chemical composition, antioxidant, anti‐Aspergillus and antigenotoxic activities. (31st January 2019)

• Record Type: Journal Article
• Authors: Aprotosoaie, Ana Clara; Miron, Anca; Ciocârlan, Nina; Brebu, Mihai; Roşu, Crăiţa Maria; Trifan, Adriana; Vochiţa, Gabriela; Gherghel, Daniela; Luca, Simon Vlad; Niţă, Alexandru; Costache, Irina‐Iuliana; Mihai, Cosmin Teodor
• Is Part Of: Flavour and fragrance journal. Volume 34:Number 3(2019:May)
• Page Start: 175
• Page End: 186
• Publication Date: 2019-01-31
• Subjects: antigenotoxicity -- bioactivity -- chemical composition -- essential oils -- Thymus species; Flavor -- Periodicals; Odors -- Periodicals; Smell -- Periodicals; 668.54
• DOI: 10.1002/ffj.3490
• Languages: English
• ISSNs: 0882-5734

Abstract

Abstract: Thymus species are important aromatic, medicinal and culinary plants with a significant economic value. This study evaluated for the first time the chemical composition and in vitro bioactivities of the essential oils (EOs) from five Moldavian Thymus species ( T. vulgaris, T . × citriodorus, T. calcareus ) and cultivars ( T. vulgaris 'Faustini', T. citriodorus 'Aureus'). The main compounds in Thymus EOs were: thymol in T. vulgaris and T. calcareus EOs (55.44% and 55.45%, respectively), lavandulol in T . × citriodorus EO (54.27%), and geraniol in T. citriodorus 'Aureus' and T. vulgaris 'Faustini' EOs (60.31% and 31.45%, respectively). T. vulgaris and T. calcareus EOs showed the most potent antioxidant activities (EC50 =0.003 mg/mL in ABTS radical cation scavenging assay) and exhibited significant inhibitory effects against aflatoxin‐producing Aspergillus flavus fungus (MIC=0.25 μL/mL). At doses that provided micromolar concentrations of thymol, T. vulgaris and T. calcareus EOs acted genoprotective at preventive and interventional levels against H2 O2 ‐induced genomic damage in V79 cells, the former being more active (6.21% and 5.52% vs . 25.13% and 7.26% tail DNA in pre‐ and post‐treatment protocols, respectively). The genoprotective effects may be ascribed to antioxidant potential and, possibly, to stimulation of DNA repair processes. The Moldavian Thymus species are valuable resources of bioactive EOs for pharmaceutical and food industries ( T. vulgaris, T.calcareus ) but also for flavor industry and perfumery ( T . × citriodorus, T. citriodorus 'Aureus', T. vulgaris 'Faustini'). Abstract : The Moldavian Thymus species are valuable resources of bioactive EOs for pharmaceutical and food industries (T. vulgaris, T. calcareus) but also for flavor industry and perfumery (T. citriodorus, T. citriodorus 'Aureus', T. vulgaris 'Faustini'). Thymol‐rich T. vulgaris and T. calcareus essential oils showed the most potent antioxidant and antifungal activities. Besides, they acted genoprotective at preventive and interventional levels against oxidative genomic damage in V79 cells.Photomicrographs of comet length in normal (a) and treated V79 cells with genotoxic agent (b), genotoxic agent and Thymus essential oils (c, d) and Thymus essential oils (e, f)