Production and in-vitro evaluation of an enzyme formulation as a potential alternative to feed antibiotics in poultry. (May 2019)

• Record Type: Journal Article
• Authors: Suresh, Gayatri; Santos, Daniel Ubaldo; Rouissi, Tarek; Brar, Satinder Kaur; Mehdi, Youcef; Godbout, Stephane; Chorfi, Younes; Ramirez, Antonio Avalos
• Is Part Of: Process biochemistry. Volume 80(2019)
• Page Start: 9
• Page End: 16
• Publication Date: 2019-05
• Subjects: AFA antibiotic feed additives -- NSP non-starch polysaccharides -- PDB potato dextrose broth -- PDA potato dextrose agar -- AP apple pomace -- DNSA 3, 5-dinitrosalicylic acid -- RSM response surface methodology -- GIT gastrointestinal tract -- Tinlet inlet temperature -- P atomization air pressure -- Fr sample feed rate -- ANOVA analysis of variance -- RSC reducing sugar concentration; Feed enzymes -- Apple pomace fermentation -- Spray drying -- Response surface methodology -- Poultry gut simulation; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2019.01.023
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Amylase and xylanase production from apple pomace fermentation by Aspergillus niger . Optimization of parameters of spray drying by response surface methodology. In vitro test in simulated poultry gut to evaluate formulation efficacy. Reducing sugar concentration measurement at end of digestion. Higher reducing sugar concentration seen in feed supplemented with formulation. Abstract: Xylanase and amylase were produced by Aspergillus niger by solid state fermentation of apple pomace. The enzyme formulation was produced by spray drying, for which, Response surface methodology was used to optimize inlet temperature (Tinlet ), atomization air pressure (P) and sample feed rate (Fr ). Optimal conditions obtained for spray drying were Tinlet 120 °C, P 0.07 MPa, and Fr 35 ml/min. The optimized spray dried powder contained 75.3 units/g xylanase, 40.3 units/g amylase, and the humidity and outlet temperature were 5.7% and 55 °C, respectively. Poultry feed for each growth phase was supplemented with enzyme formulation (500 U/kg xylanase and 267 U/kg amylase) and subjected to digestion in an in-vitro, poultry gut model, following which, the total reducing sugar concentration (RSC) of the digesta was measured. For the in vitro digestion, an increase of 23.4%, 14.70% and 116.9% in the RSC was observed in the ileal phase for the starter, grower and finisher phase, respectively. The enzyme supplemented feeds gave a higher RSC, as compared to the control, indicating the potential capacity of the formulation to improve nutrient availability, and therefore possibly broiler performance in vitro.