A validated strip-based lateral flow assay for the confirmation of sheep-specific PCR products for the authentication of meat. (February 2016)

• Record Type: Journal Article
• Authors: Yin, Rui; Sun, Yajuan; Yu, Shuang; Wang, Yi; Zhang, Meiping; Xu, Yawei; Xue, Jian; Xu, Na
• Is Part Of: Food control. Volume 60(2016:Feb.)
• Page Start: 146
• Page End: 150
• Publication Date: 2016-02
• Subjects: PCR -- Sheep -- Strip -- Meat adulteration; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2015.07.030
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Molecular methods, such as PCR and real-time PCR, have been developed to detect species in meat and meat products. Despite good specificity and sensitivity, they are not widely implemented in food control programs due to complex operation or financial reasons. In the present study, a simple, rapid and affordable method, Sheep-PCR-Strip [Sheep specific polymerase chain reaction-Strip], was developed for the authentic identification of raw and heat-treated mutton. The assay is based on PCR amplification of sheep DNA, followed by detection of the PCR product by a strip format; the result can be read within 5 min by the naked eye. There is a real advantage of the strip approach rather in the reduced time (5 min versus electrophoresis) and avoidance of chemicals (e.g. ethidiumbromide). The sensitivity of the Sheep-PCR-Strip test was established to be 0.01% for the detection of adulterated meat; the limit of detection (LOD) was up to 0.01 pg of sheep DNA. The assay was also specific for sheep, and no cross-reactions were observed in other non-target species. It is a promising new tool for sheep identification and can be rapidly modified for other meat detection and widely used for solving problems related to food quality assurance, species authentication and traceability. Highlights: A simple and rapid strip format for detection of sheep DNA was developed. The specificity of the test was validated when tested on sheep and unrelated species. The limit of detection was up to 0.01 pg of sheep DNA. The PCR product was visualized on the strip within 5 min.