Development of a novel sensitive molecularly imprinted polymer sensor based on electropolymerization of a microporous-metal-organic framework for tetracycline detection in honey. (January 2016)

• Record Type: Journal Article
• Authors: Bougrini, Madiha; Florea, Anca; Cristea, Cecilia; Sandulescu, Robert; Vocanson, Francis; Errachid, Abdelhamid; Bouchikhi, Benachir; El Bari, Nezha; Jaffrezic-Renault, Nicole
• Is Part Of: Food control. Volume 59(2016:Jan.)
• Page Start: 424
• Page End: 429
• Publication Date: 2016-01
• Subjects: Electrochemical sensor -- Molecularly imprinted polymer -- Electropolymerization -- Gold nanoparticles -- Tetracycline -- Honey; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2015.06.002
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Electrochemical sensor for the detection of tetracycline, based on a gold electrode surface modified with a molecularly imprinted polymer microporous-metal-organic framework, is described. Imprinted gold nanoparticles composites are assembled on Au surfaces by the electropolymerization of p -aminothiophenol functionalized gold nanoparticles in the presence of the imprint molecule. The electrochemical sensor was characterized by linear sweep voltammetry in the presence of hexacyanoferrate/hexacyanoferrite as redox probe. In order to achieve optimal preconcentration, the influence of various parameters such as extraction and incubation times, washing and eluting solutions was evaluated. The sensor showed a linear range from 224 fM to 22.4 nM and a limit of detection of 0.22 fM. The proposed method was successfully applied to determine tetracycline in honey. The recoveries of tetracycline are in the range of 101.8 %−106.0 % with an RSD ≤8.3%. Highlights: We developed a biosensor based on molecularly imprinted polymer (MIP) microporous metal-organic framework (MMOF). The MIP was prepared by electro-polymerization of p -aminothiophenol functionalized gold nanoparticles (Au-NPs). The biosensor was used for the determination of Tetracycline (TC) in honey. The limit of detection (LOD) was 2.24 × 10 −16 M. The recoveries of TC are in the range of 101.8 %−106.0 % with a relative standard deviation (RSD) ≤ 8.3%.