Development of antifungal ingredients for dairy products: From in vitro screening to pilot scale application. (August 2019)

• Record Type: Journal Article
• Authors: Garnier, Lucille; Mounier, Jérôme; Lê, Sébastien; Pawtowski, Audrey; Pinon, Nicolas; Camier, Bénédicte; Chatel, Manon; Garric, Gilles; Thierry, Anne; Coton, Emmanuel; Valence, Florence
• Is Part Of: Food microbiology. Volume 81(2019)
• Page Start: 97
• Page End: 107
• Publication Date: 2019-08
• Subjects: Fungal spoilage -- Biopreservation -- Lactic acid bacteria -- Propionibacteria -- Fungi; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2018.11.003
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: Biopreservation represents a complementary approach to traditional hurdle technologies for reducing microbial contaminants (pathogens and spoilers) in food. In the dairy industry that is concerned by fungal spoilage, biopreservation can also be an alternative to preservatives currently used (e.g. natamycin, potassium sorbate). The aim of this study was to develop antifungal fermentates derived from two dairy substrates using a sequential approach including an in vitro screening followed by an in situ validation. The in vitro screening of the antifungal activity of fermentates derivating from 430 lactic acid bacteria (LAB) (23 species), 70 propionibacteria (4 species) and 198 fungi (87 species) was performed against four major spoilage fungi ( Penicillium commune, Mucor racemosus, Galactomyces geotrichum and Yarrowia lipolytica ) using a cheese-mimicking model. The most active fermentates were obtained from Lactobacillus brevis, Lactobacillus buchneri, Lactobacillus casei/paracasei and Lactobacillus plantarum among the tested LAB, Propionibacterium jensenii among propionibacteria, and Mucor lanceolatus among the tested fungi. Then, for the 11 most active fermentates, culture conditions were optimized by varying incubation time and temperature in order to enhance their antifungal activity. Finally, the antifungal activity of 3 fermentates of interest obtained from Lactobacillus rhamnosus CIRM-BIA1952, P. jensenii CIRM-BIA1774 and M. lanceolatus UBOCC-A-109193 were evaluated in real dairy products (sour cream and semi-hard cheese) at a pilot-scale using challenge and durability tests. In parallel, the impact of these ingredients on organoleptic properties of the obtained products was also assessed. In semi-hard cheese, application of the selected fermentates on the cheese surface delayed the growth of spoilage molds for up to 21 days, without any effect on organoleptic properties, P. jensenii CIRM-BIA1774 fermentate being the most active. In sour cream, incorporation of the latter fermentate at 2 or 5% yielded a high antifungal activity but was detrimental to the product organoleptic properties. Determination of the concentration limit, compatible with product acceptability, showed that incorporation of this fermentate at 0.4% prevented growth of fungal contaminants in durability tests but had a more limited effect against M. racemosus and P. commune in challenge tests. To our knowledge, this is the first time that the workflow followed in this study, from in vitro screening using dairy matrix to scale-up in cheese and sour cream, is applied for production of natural ingredients relying on a large microbial diversity in terms of species and strains. This approach allowed obtaining several antifungal fermentates which are promising candidates for dairy products biopreservation. Highlights: Microbial dairy fermentates, can constitute a natural alternative to the use of chemical preservatives such as natamycine in dairy industry. A sequential approach combining in vitro and in situ tests allows the screening of 698 microorganisms of food interest and leads to 3 promising antifungal ingredients. Dairy-based ingredients fermented by 3 strains of Propionibacterium jensenii, Lactobacillus rhamnosus and Mucor lanceolatus respectively, possess antifungal activity in real dairy products. Fermentate derived from Propionibacterium jensenii CIRM-BIA952 combined with Mucor lanceolatus UBOCC-A-09193 delays the growth of spoilage molds for up to 21 days on semi-hard cheese. The microbial antifungal activity is strongly species, strain and substrate used dependent.