Rapid detection of cow milk adulteration/contamination in goat milk by a lateral flow colloidal gold immunoassay strip. Issue 1 (7th February 2019)

• Record Type: Journal Article
• Authors: Liu, Bochao; Si, Jinhong; Zhao, Fang; Wang, Qi; Wang, Yu; Li, Jinfeng; Li, Chengyao; Li, Tingting
• Is Part Of: Journal of dairy research. Volume 86:Issue 1(2019)
• Page Start: 94
• Page End: 97
• Publication Date: 2019-02-07
• Subjects: Colloidal gold nanoparticles, -- contamination or adulteration, -- cow milk, -- goat milk, -- lateral flow immunoassay strip; Dairying -- Periodicals; Dairying -- Research -- Periodicals; 636.214205
• DOI: 10.1017/S0022029919000116
• Languages: English
• ISSNs: 0022-0299

Abstract

Abstract: Current available methods to detect cow milk adulteration or accidental contamination of goat milk are both laborious and time consuming. The aim of this technical research communication was to develop a simple, rapid, specific and sensitive method for quantitative detection of cow milk in goat milk. A competitive lateral flow immunoassay (LFIA) strip was developed using a specific monoclonal antibody (mAb) labeled with colloidal gold nanoparticles (GNPs) for specifically binding to cow milk casein. The detection limit of this rapid detection was 0.07% of cow milk in goat milk, providing equal specificity and higher sensitivity when compared with a commercial enzyme-linked immunosorbent assay (ELISA). These result suggest that the established rapid GNPs-LFIA strip could be used for monitoring cow milk adulteration/contamination of goat milk.