Mitochondrial activity as an indicator of fish freshness. (30th July 2019)

• Record Type: Journal Article
• Authors: Cléach, Jérôme; Pasdois, Philippe; Marchetti, Philippe; Watier, Denis; Duflos, Guillaume; Goffier, Emmanuelle; Lacoste, Anne-Sophie; Slomianny, Christian; Grard, Thierry; Lencel, Philippe
• Is Part Of: Food chemistry. Volume 287(2019)
• Page Start: 38
• Page End: 45
• Publication Date: 2019-07-30
• Subjects: Rhodamine 123 (PubChem CID: 9929799) -- Carbonyl cyanide 3-chlorophenylhydrazone (PubChem CID: 2603) -- Tris (hydroxymethyl)aminomethane (PubChem CID: 6503) -- Ethylene-bis(oxyethylenenitrilo)tetraacetic acid (PubChem CID: 6207) -- 4-Morpholinepropanesulfonic acid (PubChem CID: 70807) -- Malate (PubChem CID: 525) -- Succinate (PubChem CID 160419) -- Glutamate (PubChem CID: 33032) -- Adenosine 5′-diphosphate (PubChem CID: 128882) -- Carboxyatractyloside (PubChem CID: 20055804) -- Cytochrome c (PubChem CID 16057918) -- Potassium chloride (PubChem CID: 4873) -- Sucrose (PubChem CID: 5988) -- Magnesium chloride (PubChem CID: 5360315) -- Potassium dihydrogen phosphate (PubChem CID: 516951) -- Dimethyl sulfoxide (PubChem CID: 679) -- Glutaraldehyde (PubChem CID: 3485) -- Sodium cacodylate (PubChem CID: 2724247) -- Osmium tetroxide (PubChem CID: 30318) -- Uranyl acetate (PubChem CID: 10915) -- Acetonitrile (PubChem CID: 6342); Mitochondrial membrane potential (ΔΨm) -- Mitochondrial respiration -- Fish freshness -- Gilthead sea bream (Sparus aurata); Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.02.076
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Mitochondria function as reliable indicator of fish freshness. Several ultrastructural post mortem damages visible in fish fillets mitochondria. Mitochondrial respiration altered in fish fillets from 96 h stored at +4 °C. Mitochondrial potential (ΔΨm ) significantly disrupted after 96 h of storage at +4 °C. Abstract: The current methods used to routinely assess freshness in the fishing industry reflect more a state of spoilage than a state of freshness. Mitochondria, the seat of cellular respiration, undergo profound changes in post mortem tissues. The objective of this study was to demonstrate that mitochondrial activity constitutes a putative early fish freshness marker. The structure of gilthead sea bream ( Sparus aurata ) muscle tissue was evaluated over time by transmission electron microscopy. Respiration was assessed in mitochondria isolated from sea bream fillets using oxygraphy. Membrane potential (ΔΨm ) was determined by fluorescence (Rhodamine 123). Mitochondrial activity of fillets stored at +4 °C was studied for 6 days. Changes in mitochondrial cristae structure appeared from Day 3 highlighting the presence of dense granules. ΔΨm and mitochondrial activity were significantly disrupted in sea bream fillets after 96 h of storage at +4 °C. Mitochondrial activity constituted a reliable and early indicator of fish freshness.