Development of a broad-spectrum monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for the multi-residue detection of avermectins in edible animal tissues and milk. (15th July 2019)

• Record Type: Journal Article
• Authors: Ni, Tengteng; Peng, Dapeng; Wang, Yanxin; Pan, Yuanhu; Xie, Shuyu; Chen, Dongmei; Wang, Yulian; Tao, Yanfei; Yuan, Zonghui
• Is Part Of: Food chemistry. Volume 286(2019)
• Page Start: 234
• Page End: 240
• Publication Date: 2019-07-15
• Subjects: Avermectins -- Monoclonal antibody -- Indirect competitive enzyme-linked immunosorbent assay -- Edible animal tissues -- Milk; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.02.011
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlight: A sensitive and broad-spectrum mAb against 4 avermectins was obtained. Molecular modelling was used to hapten design and antibody analyze. A mAb-based ic-ELISA was developed for avermectins in various samples. Abstract: Avermectins (AVMs) are a group of anti-parasitic agents that have been widely used in food-producing animals. To monitor the residue of the AVMs, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed with a simple sample preparation procedure. Conjugates of 4″-HS-IVM/AVM on three different proteins were used to raise a broad-spectrum monoclonal antibody (mAb), 6D4, that had IC50 values for avermectin, ivermectin, eprinomectin and emamectin of 7.2, 10.4, 19.8 and 20.8 µg L −1, respectively. The limit of detection and limit of quantitation of this method for AVMs in various matrix samples ranged from 0.5 to 5.4 µg L −1 and 1.0 to 10.3 µg L −1, respectively. The recoveries of the samples spiked with AVMs were in the range of 78.1–110.5% with coefficients of variation below 14%. The ic-ELISA was applied to monitor ivermectin in the milk samples, and the results showed a good correlation with that of HPLC-MS/MS.