Isolation and optical properties of epimerically pure bacteriochlorophyll-f homologs. (May 2019)

• Record Type: Journal Article
• Authors: Kinoshita, Yusuke; Harada, Jiro; Mizoguchi, Tadashi; Tamiaki, Hitoshi
• Is Part Of: Dyes and pigments. Volume 164(2019)
• Page Start: 267
• Page End: 271
• Publication Date: 2019-05
• Subjects: Chlorosome -- Fluorescence emission -- Green sulfur bacterium -- High-performance liquid chromatography -- Visible absorption; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2019.01.041
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: Epimerically and homologously pure farnesyl bacteriochlorophylls- f (BChls- f F ) were isolated from the cultured cells of the bchU gene-deleted mutant of Chlorobaculum limnaeum, which is a green sulfur bacterium, by reverse-phase HPLC. Homologously pure 8-ethyl-, propyl-, and isobutyl-BChls- f F with the 12-ethyl group were fully characterized by their VIS, 1 H NMR, and MS spectra. All the 3 1 -epimerically pure samples of each homolog showed nearly the same visible absorption and fluorescence emission spectra in THF, and their emission quantum yields and lifetimes were independent of the 3 1 -stereochemistry and 8 2 -methylation. Graphical abstract: Image 1 Highlights: Epimerically and homologously pure farnesyl bacteriochlorophylls- f were isolated. Homologously pure samples were fully characterized by VIS, 1 H NMR, and MS spectra. All epimers showed almost same VIS absorption and fluorescence emission spectra. Emission quantum yields and lifetimes were independent on their substituents.