Isolation and optical properties of epimerically pure bacteriochlorophyll-f homologs. (May 2019) - Kinoshita, Yusuke
Abstract: Epimerically and homologously pure farnesyl bacteriochlorophylls- f (BChls- f F ) were isolated from the cultured cells of the bchU gene-deleted mutant of Chlorobaculum limnaeum, which is a green sulfur bacterium, by reverse-phase HPLC. Homologously pure 8-ethyl-, propyl-, and isobutyl-BChls- f F with the 12-ethyl group were fully characterized by their VIS, 1 H NMR, and MS spectra. All the 3 1 -epimerically pure samples of each homolog showed nearly the same visible absorption and fluorescence emission spectra in THF, and their emission quantum yields and lifetimes were independent of the 3 1 -stereochemistry and 8 2 -methylation. Graphical abstract: Image 1 Highlights: Epimerically and homologously pure farnesyl bacteriochlorophylls- f were isolated. Homologously pure samples were fully characterized by VIS, 1 H NMR, and MS spectra. All epimers showed almost same VIS absorption and fluorescence emission spectra. Emission quantum yields and lifetimes were independent on their substituents.