Immunomodulatory activity of a water-soluble polysaccharide obtained from highland barley on immunosuppressive mice models. Issue 1 (21st December 2018)

• Record Type: Journal Article
• Authors: Han, Lirong; Meng, Meng; Guo, Mingzhu; Cheng, Dai; Shi, Lei; Wang, Xu; Wang, Chunling
• Is Part Of: Food & function. Volume 10:Issue 1(2019)
• Page Start: 304
• Page End: 314
• Publication Date: 2018-12-21
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c8fo01991f
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : Immunomodulatory activity of a water-soluble polysaccharide obtained from highland barley on immunosuppressive mice models. Abstract : A water-soluble polysaccharide (BP-1) was extracted and purified from highland barley ( Hordeum vulgare L.) and its average molecular weight was about 6.7 × 10 4 Da. In this study, the immunomodulatory activity of BP-1 on the immunosuppressive BALB/c mice model and its molecular mechanism were elucidated. It was found that the weight indexes of spleen and thymus were significantly increased by BP-1 (80 mg kg −1 and 160 mg kg −1 ) treatment in the immunosuppressive mice model. The results showed that BP-1 (80 mg kg −1 and 160 mg kg −1 ) could significantly increase the number of bone marrow cells (BMC) and peripheral blood white blood cells (WBC) in the immunosuppressive mice model. In addition, the result further confirmed that BP-1 could increase the serum levels of IL-2, TNF-α and IFN-γ, so as to improve the immune function of immunosuppressive mice. The results showed that BP-1 (80 mg kg −1 and 160 mg kg −1 ) could promote the proliferation of spleen cells and the natural killer (NK) cell activity in vivo . The quantitative real-time polymerase chain reaction (qRT-PCR) and ELISA results revealed that BP-1 (80 mg kg −1 and 160 mg kg −1 ) could enhance the production of IL-2, TNF-α, IFN-γ, IgG and IgM in the spleen of immunosuppressive mice. The HE (hematoxylin and eosin) stained histopathological images showed that BP-1 (80 mg kg −1 and 160 mg kg −1 ) could repair the damage induced by CTX in the spleen cells of immunosuppressive mice. The result of macrophages showed that BP-1 (80 mg kg −1 and 160 mg kg −1 ) could promote the proliferation and phagocytosis activity of macrophages in immunosuppressive mice. Furthermore, BP-1 could activate macrophages by the TLR-4, TRAF6, TAK1 and nuclear factor κB (NF-κB) p65 pathways in vivo . These results suggested that BP-1 has a remarkable immunomodulatory activity on the immunosuppressive mice model.