Fabrication of a core-shell-shell magnetic polymeric microsphere with excellent performance for separation and purification of bromelain. (15th June 2019)

• Record Type: Journal Article
• Authors: Han, Juan; Wang, Li; Wang, Lei; Li, Chunmei; Mao, Yanli; Wang, Yun
• Is Part Of: Food chemistry. Volume 283(2019)
• Page Start: 1
• Page End: 10
• Publication Date: 2019-06-15
• Subjects: Bromelain -- Magnetic polymeric microspheres -- Separation and purification -- Core-shell-shell structure; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.01.016
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: A core-shell-shell structure magnetic polymeric microsphere was designed for the first time. The microspheres showed excellent performance for separation and purification of bromelain. The microspheres have the capability of thermal protection to the enzyme. The microspheres exhibited high adsorption capacity, good stability and acceptable reusability. Abstract: Bromelain is an important industrial proteolytic enzyme which has great commercial value and is of wide application in food, beverage, tenderization, cosmetic, textile and pharmaceutical industries. In this work, the core-shell-shell magnetic polymeric microspheres (Fe3 O4 @SiO2 @P(NIPAM-co-AIM)/Ni 2+ ) composed of an SiO2 -coated Fe3 O4 magnetite core and a Ni 2+ -immobilized cross-linked poly (N-isopropyl acrylamide-co-propylimidazole) (NIPAM-co-AIM) shell were synthesized via distillation-precipitation polymerization. The Ni 2+ cations in the magnetic polymeric microspheres shell provided docking sites for histidine and the microspheres exhibited excellent performance in the separation of bromelain with a binding capacity as high as 198 mg/g, and the recovery of enzyme activity could achieve 80%. It was found that the microspheres showed excellent performance for separation and purification of bromelain from the crude extract of pineapple peel, moreover the enzyme structure remained unchanged before and after elution process.