Bioavailability of docosahexaenoic acid 22:6(n-3) from enantiopure triacylglycerols and their regioisomeric counterpart in rats. (15th June 2019)

• Record Type: Journal Article
• Authors: Linderborg, Kaisa M.; Kulkarni, Amruta; Zhao, Ai; Zhang, Jian; Kallio, Heikki; Magnusson, Johann D.; Haraldsson, Gudmundur G.; Zhang, Yumei; Yang, Baoru
• Is Part Of: Food chemistry. Volume 283(2019)
• Page Start: 381
• Page End: 389
• Publication Date: 2019-06-15
• Subjects: TAGs triacylglycerols -- DHA docosahexaenoic acid -- LC (n-3) PUFA long chain (n-3) polyunsaturated fatty acids -- CAL Candida antarctica lipase -- sn-1 DHA 2, 3-distearoyl-1-docosahexaenoyl-sn-glycerol -- sn-2 DHA 1, 3-distearoyl-2-docosahexaenoyl-sn-glycerol -- sn-3 DHA 1, 2-distearoyl-3-docosahexaenoyl-sn-glycerol -- TS tristearin -- NF standard AIN-93G fed group -- GC gas chromatography -- PL phospholipid -- FAME fatty acid methyl ester -- ANOVA Analysis of Variance -- MUFA monounsaturated fatty acid -- SFA saturated fatty acid -- ARA arachidonic acid -- ALA α-linolenic acid -- EPA eicosapentaenoic acid -- LA linoleic acid -- SD standard deviation; Absorption -- Docosahexaenoic acid -- (n-3) PUFAs -- Plasma and fecal lipids -- Stearic acid -- Enantiospecificity -- Regiospecificity -- Structured lipids; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.12.130
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: DHA was efficiently absorbed in Sprague-Dawley rats with mild n-3 deficiency. No significant differences in the absorption between DHA in sn -1 and sn -3 positions. Less fecal DHA from the sn -2 position compared with the sn -1 and sn -3 positions. 5-Day intervention enough to modify the fatty acid profile of plasma phospholipids. Abstract: Lack of synthetic enantiospecific triacylglycerols (TAGs) has hindered our understanding of the impact of TAG structure on the absorption and metabolic fate of fatty acids (FAs). In a five-day feeding trial with mildly (n-3) deficient rats, the bioavailability of docosahexaenoic acid [22:6(n-3), DHA] and stearic acid (18:0) from the two different enantiomers of TAG: sn -22:6(n-3)-18:0-18:0 and sn -18:0-18:0-22:6(n-3), and their regioisomeric TAG: sn -18:0-22:6(n-3)-18:0 was compared. Less secretion of fecal DHA was detected from the sn -2 position compared with the sn -1 and sn -3 positions, but no difference was found in DHA content of the fasting plasma or in the weight of the body or organs. 18:0 was lost to feces mainly as cleaved from the primary positions but also as glycerol-bound. The 5-day intervention in rats was long enough to modify the fatty acid profile of plasma phospholipids.