Heteropolysaccharide-producing bifidobacteria for the development of functional dairy products. (March 2019)

• Record Type: Journal Article
• Authors: Llamas-Arriba, María Goretti; Peirotén, Ángela; Puertas, Ana Isabel; Prieto, Alicia; López, Paloma; Pardo, Miguel Á.; Rodríguez, Eva; Dueñas, María Teresa
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 102(2019)
• Page Start: 295
• Page End: 303
• Publication Date: 2019-03
• Subjects: Bifidobacteria -- Exopolysaccharide -- Immunomodulation -- Zebrafish -- Technological properties -- Adhesion; DMEM Dulbecco's Modified Eagle medium -- DSS dextran sodium sulphate -- EDTA ethylenediaminetetraacetic acid -- EPS exopolysaccharides -- EW embryo water -- HePS heteropolysaccharides -- HoPS homopolysaccharides -- HPLC-SEC high-performance size exclusion liquid chromatography -- IR infrared -- LAB lactic acid bacteria -- mTSB modified tryptic soy broth -- LPS lipopolysaccharide -- MEM-Alpha minimum essential medium-alpha -- p-GTF priming-glycosyltransferase -- PMA phorbol-12-myristate-13-acetate -- PMA-THP-1 THP-1 monocytes differentiated to macrophages with PMA -- RCM reinforced clostridial medium -- RPMI Roswell Park memorial institute medium -- RT room temperature -- TEM transmission electron microscopy -- TFA trifluoroacetic acid; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2018.12.044
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Bifidobacterium longum INIA P132 and Bifidobacterium infantis INIA P731, isolated from infant-faeces, were investigated in this work. Regarding the probiotic and technological potential of the bifidobacteria, both were resistant to gastrointestinal tract simulated conditions. B. longum showed high survival upon freezing and thawing as well as lyophilisation and was able to grow in milk. B. infantis had higher adhesion capacity to human Caco-2 cells than the commercial probiotic Bifidobacterium animalis BB12 strain. Moreover, both bacteria secrete heteropolysaccharides (HePS) composed of rhamnose, galactose and glucose. In a dextran sodium sulphate-induced enterocolitis model in zebra fish larvae, treatment with each HePS preparation resulted in a decrease of the larval mortality. In addition, the HePS from B. longum immunomodulated in vitro human macrophages treated with the inflammatory Escherichia coli O111:B4 lipopolysaccharide. Thus, both studied bifidobacteria and their HePS have potential beneficial effects on health and thus, to their application in functional foods. Highlights: B. longum INIA P132 showed good stability as frozen and freeze-dried culture and was able to grow in milk. B. infantis INIA P731 adhered highly to human Caco-2 cells and survived GI conditions. EPS of B. longum INIA P132 and B. infantis INIA P731 were partially characterised. Both HePS reduced larvae mortality in a DSS-induced enterocolitis zebrafish model. B. longum INIA P132 EPS immunomodulated in vitro human macrophages treated with LPS.