Purification of β‐carotene 15, 15′‐monooxygenase from pig intestine and its enzymatic hydrolysis of pigment in soybean oil. (2nd November 2018)

• Record Type: Journal Article
• Authors: Yu, Dianyu; Zhang, Ruchun; Wang, Yuqi; Zou, Dezhi; Li, Tingting; Tang, Honglin; Jiang, Lianzhou; Wang, Liqi
• Is Part Of: International journal of food science & technology. Volume 54:Number 2(2019)
• Page Start: 480
• Page End: 489
• Publication Date: 2018-11-02
• Subjects: Enzymolysis -- protein solution -- purification -- soybean oil -- β‐carotene -- β‐carotene 15, 15′‐monooxygenase; Food industry and trade -- Periodicals; 664
• DOI: 10.1111/ijfs.13960
• Languages: English
• ISSNs: 0950-5423

Abstract

Summary: β‐Carotene 15, 15′‐monooxygenase was isolated and purified from the intestinal mucosa of pigs, and then, it was applied to hydrolyse the pigment in soybean oil, and thus, vitamin A fortified soybean oil was obtained. The pig intestinal mucosal protein solution was purified to a specific activity of 2.487 × 10 −4 U mg −1, maximum reaction velocity ( V max ) of 8.42 × 10 −9 mol/h and Michaelis constant (Km ) of 2.03 × 10 −5 m . The protein solution had a molecular mass of 156 kDa by gel filtration. The sodium deoxycholate concentrations, optimum pH, Tween 40 amount and enzyme amount for vitamin A production in soybean oil were determined to be 6.0 mm, 8.0, 3.0% (w/v) and 0.2 U/mL enzyme, respectively. Under these conditions, β‐carotene 15, 15′‐monooxygenase produced 14.65 mg/L vitamin A after 20 h, with a conversion yield of β‐carotene of 33.29% (w/w). Therefore, the nutrients in soybean oil were improved. Abstract : β‐carotene 15, 15′‐monooxygenase was isolated and purified from the intestinal mucosa of pigs, and then, it was applied to hydrolyse the pigment in soybean oil, and thus, vitamin A fortified soybean oil was obtained.