Broad-spectrum monoclonal antibody and a sensitive multi-residue indirect competitive enzyme-linked immunosorbent assay for the antibacterial synergists in samples of animal origin. (15th May 2019)

• Record Type: Journal Article
• Authors: Han, Xiaoya; Sheng, Feng; Kong, Dexin; Wang, Yulian; Pan, Yuanhu; Chen, Mo; Tao, Yanfei; Liu, Zhenli; Ahmed, Saeed; Yuan, Zonghui; Peng, Dapeng
• Is Part Of: Food chemistry. Volume 280(2019)
• Page Start: 20
• Page End: 26
• Publication Date: 2019-05-15
• Subjects: Antibacterial synergists -- Broad-spectrum -- Monoclonal antibody -- Multi-residue -- Indirect competitive enzyme-linked immunosorbent assay -- Food-producing animals; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.12.040
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlight: Molecular modelling was used for hapten design and antibody analysis. A sensitive and broad-spectrum mAb against 6 antibacterial synergists was obtained. A mAb-based ic-ELISA was developed for antibacterial synergists in various samples. Abstract: To monitor the abuse of antibacterial synergists, a hapten, trimethoprim carboxylic derivative (TMPCOOH), was designed by using molecular modelling technology. A broad-spectrum monoclonal antibody (mAb) TMP/2G1 was prepared, for which the IC50 values of trimethoprim, diaveridine, aditoprim, baquiloprim, ormetoprim, and brodimoprim were 0.232, 0.527, 1.479, 4.354, 0.965, and 0.119 µg L −1, respectively. Based on the broad spectrum mAb, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed to determine the residues of antibacterial synergists. The limit of detection regarding the developed ic-ELISA for antibacterial synergists ranged from 0.025 to 1.126 µg L −1 in milk, honey and edible animal tissues. The recoveries ranged from 81.4% to 107.7%, with a coefficient of variation less than 20%. A good correlation ( R 2 = 0.994) between the ic–ELISA and HPLC–MS/MS showed the reliability of the developed ic-ELISA.