Glycerol increases growth, protein production and alters the fatty acids profile of Spirulina (Arthrospira) sp LEB 18. (January 2019)

• Record Type: Journal Article
• Authors: Morais, Etiele Greque de; Druzian, Janice Izabel; Nunes, Itaciara Larroza; Morais, Michele Greque de; Costa, Jorge Alberto Vieira
• Is Part Of: Process biochemistry. Volume 76(2019)
• Page Start: 40
• Page End: 45
• Publication Date: 2019-01
• Subjects: Cyanobacteria -- Glycerol -- Biomass increase -- Mixotrophic; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2018.09.024
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Addition of glycerol to the culture altered the fatty acid profile of Spirulina sp. LEB 18. The protein content increased in proportion to the glycerol concentration in the medium. Spirulina sp. LEB 18 growth increased after glycerin addition. Abstract: Most of the crude glycerol produced globally is generated by biodiesel production, which makes this byproduct an environmental responsibility of the biofuel industries. Among the forms of this compound in use, microalgae cultivation is a promising alternative that may generate a reduction in crude glycerol treatment costs via using it as an organic, carbon-rich substrate in culture media. In this work, the influence of different concentrations of glycerol in the culture medium, the composition of fatty acids and proteins in Spirulina sp. LEB 18 biomass and their effect on its growth were investigated. The fatty acid profile of the biomass was altered, showing a 20% increase in the unsaturated concentration and a 60% reduction in the saturated concentration in the culture supplemented with 0.05 mol L −1 of glycerol compared to those in the control. The addition of the substrate stimulated an increase in its cellular concentration (3.00 g L −1, 0.05 mol L −1 ), productivity (0.72 g L −1 d −1, 0.05 mol L −1 ) and its protein production (69.78% w w −1, 0.05 mol L −1 ).