Simple column-switching ion chromatography method for determining eight monosaccharides and oligosaccharides in honeydew and nectar. (1st March 2016)

• Record Type: Journal Article
• Authors: Ni, Chengzhu; Zhu, Binhe; Wang, Nani; Wang, Muhua; Chen, Suqing; Zhang, Jiajie; Zhu, Yan
• Is Part Of: Food chemistry. Volume 194(2016)
• Page Start: 555
• Page End: 560
• Publication Date: 2016-03-01
• Subjects: Ion chromatography -- Sugars -- Honeydew -- Nectar -- Column-switching; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.08.049
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A column-switching ion chromatography method was used to analyze 8 sugars. Organics in honeydew and nectar was removed by on-line elimination process. Honeydew had much more oligosaccharides than nectar. The method may be used to analyze other sugars or ions in various organic matrices. Abstract: Honeydew is excreted by aphids as a sweet waste and nectar is floral honey. Honeydew and nectar are complicated samples which consist of various sugars and amino acids. In this work, a simple ion chromatography with column-switching method was developed for the simultaneous analysis of 8 monosaccharides and oligosaccharides in honeydew and nectar. A reversed-phase column was used as a pretreatment column to eliminate organics on-line and sugars were eluted from a collection loop to analytical column by using column-switching technique. This method showed good linearity ( r ⩾ 0.9994) and afforded low limits of detection ranging from 1.55 to 10.17 μg L −1 for all the analytes. Recoveries ranged from 95% to 105% and repeatability results were acceptable with relative standard deviation of less than 3.21% ( n = 6). This method was successfully applied to quantification of these sugars in honeydew and nectar. These results showed honeydew had much more oligosaccharides than nectar.