Competitive immunochromatographic assay for leptosperin as a plausible authentication marker of manuka honey. (1st March 2016)

• Record Type: Journal Article
• Authors: Kato, Yoji; Araki, Yukako; Juri, Maki; Ishisaka, Akari; Nitta, Yoko; Niwa, Toshio; Kitamoto, Noritoshi; Takimoto, Yosuke
• Is Part Of: Food chemistry. Volume 194(2016)
• Page Start: 362
• Page End: 365
• Publication Date: 2016-03-01
• Subjects: HPLC high-performance liquid chromatography -- ELISA enzyme-linked immunosorbent assay -- UMFHA Unique Manuka Factor Honey Association -- BSA bovine serum albumin; Immunochromatography -- Quality control -- Glycoside -- Leptospermum; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.08.040
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Leptosperin, a unique glycoside, is exclusively found in manuka honey. Competitive immunochromatography kit for leptosperin has been developed. Using the kit, the amount of leptosperin can be determined easily. Abstract: Manuka honey is known as one of the premium honeys because of its unique property: a potent antibacterial activity. Leptosperin, methyl syringate 4-O-β-d -gentiobioside, has been specifically identified in manuka honey. Because leptosperin is relatively stable under warmer conditions, measuring leptosperin levels may be applied to authenticate manuka honey. In this study, an immunochromatographic separation and quantification of leptosperin techniques have been developed. The concentration of leptosperin measured by immunochromatography was significantly correlated with the concentration measured by high-performance liquid chromatography (HPLC) or enzyme-linked immunosorbent assay (ELISA). Because the immunochromatographic method is rapid and reliable, it could be applied to on-site quality control or inspection of honey samples by a beekeeper, a manufacturer, an inspector, a retailer, or a consumer.