Optimisation of pressurised water extraction of polysaccharides from blackcurrant and its antioxidant activity. (1st March 2016)

• Record Type: Journal Article
• Authors: Xu, Yaqin; Cai, Fei; Yu, Zeyuan; Zhang, Ling; Li, Xingguo; Yang, Yu; Liu, Gaijie
• Is Part Of: Food chemistry. Volume 194(2016)
• Page Start: 650
• Page End: 658
• Publication Date: 2016-03-01
• Subjects: d-Glucose (PubChem CID: 5793) -- d-Galacturonic acid (PubChem CID: 84740) -- Trifluoroacetic acid (PubChem CID: 6422) -- Phenanthroline (PubChem CID: 1318) -- Pyrogallol (PubChem CID: 1057) -- 1, 1-Diphenyl-1-picrylhydrazyl (PubChem CID: 2735032) -- Ascorbic acid (PubChem CID: 54670067); Blackcurrant -- Polysaccharides -- Pressurised water extraction -- Response surface methodology -- Chemical characterisation -- Antioxidant activity; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.08.061
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Polysaccharides were extracted from blackcurrant using an eco-friendly method. Experimental conditions of the pressurised water extraction were optimised by RSM. Pressurised water extraction produced higher yields than hot water extraction. Polysaccharides from blackcurrant exhibited strong antioxidant activity in vitro . Abstract: Pressurised water extraction (PWE) of polysaccharides from blackcurrant fruits was investigated using a response surface methodology (RSM). The optimal conditions for PWE were: time 51 min, pressure 1.6 MPa, and temperature 52 °C. Under these conditions, the experimental yield of Ribes nigrum L. polysaccharides (RNLP) was 11.68 ± 0.12%, which closely agreed with the predicted value (11.77%). After preliminary purification with D4006 macroporous resin, RNLP I was obtained and its chemical characterisation was undertaken by GC, HPLC, and IR spectroscopy. RNLP I was composed of rhamnose, arabinose, xylose, mannose, galactose, and glucose with a molar ratio of 2.89:14.82:1.02:1.00:2.53:6.39 and its molecular weight was 1.49 × 10 4 kDa. The antioxidant activity of RNLP I was evaluated by free radical scavenging assays and a reducing power assay in vitro . RNLP I showed strong DPPH and superoxide radical scavenging activities and reducing power.