In vitro and in vivo biological activities of anthocyanins from Nitraria tangutorun Bobr. fruits. (1st March 2016)

• Record Type: Journal Article
• Authors: Ma, Tao; Hu, Na; Ding, Chenxi; Zhang, Qiulong; Li, Wencong; Suo, Yourui; Wang, Honglun; Bai, Bo; Ding, Chenxu
• Is Part Of: Food chemistry. Volume 194(2016)
• Page Start: 296
• Page End: 303
• Publication Date: 2016-03-01
• Subjects: Anthocyanins -- Nitraria tangutorun Bobr. -- Antioxidant activities -- In vitro and in vivo -- Enrichment; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.07.110
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: The optimal conditions for enrichment and purification of anthocyanins are determined. The anthocyanins have better scavenging effects on DPPH and OH in vitro than vitamin C. The anthocyanins are related to improve blood lipid metabolism of hyperlipemia rats. The anthocyanins are related to increase the SOD activity and the TAC of hyperlipemia rats. Abstract: Anthocyanins are the main compounds in Nitraria tangutorun Bobr. The enrichment and purification of anthocyanins on macroporous resins were investigated. Regarding anthocyanin purification, static adsorption and desorption were studied. The optimal experimental conditions were the following: resin type: X-5; static adsorption time: 6 h; desorption solution: ethanol–water–HCl (80:19:1, V/V/V; pH 1); desorption time: 40 min. Furthermore, the in vitro and in vivo biological activities of the anthocyanins were evaluated. The anthocyanins showed ideal scavenging effects on free radicals in vitro, especially on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl free radical ( OH). In the animal experiment, blood lipid metabolism of hyperlipidemia rats was regulated by anthocyanin contents. The superoxide dismutase (SOD) activity and the total antioxidant capacity (TAC) of hyperlipidemia rats were also improved by anthocyanins. These results showed that anthocyanins from N. tangutorun Bobr. fruits had potential biological activities in vivo as well as in vitro .