Development of a fast DNA extraction method for sea food and marine species identification. (15th July 2016)

• Record Type: Journal Article
• Authors: Tagliavia, Marcello; Nicosia, Aldo; Salamone, Monica; Biondo, Girolama; Bennici, Carmelo Daniele; Mazzola, Salvatore; Cuttitta, Angela
• Is Part Of: Food chemistry. Volume 203(2016)
• Page Start: 375
• Page End: 378
• Publication Date: 2016-07-15
• Subjects: Marine species -- DNA extraction -- PCR amplification -- DNA barcoding -- Mislabeled seafood -- Species identification; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.02.095
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A DNA extraction method for the rapid sea food identification is proposed. The method proven effective also on transformed food. No DNA purification step is required for immediate PCR analysis. Cost effective of large scale analysis is ensured. Abstract: The authentication of food components is one of the key issues in food safety. Similarly taxonomy, population and conservation genetics as well as food web structure analysis, also rely on genetic analyses including the DNA barcoding technology. In this scenario we developed a fast DNA extraction method without any purification step from fresh and processed seafood, suitable for any PCR analysis. The protocol allows the fast DNA amplification from any sample, including fresh, stored and processed seafood and from any waste of industrial fish processing, independently of the sample storage method. Therefore, this procedure is particularly suitable for the fast processing of samples and to carry out investigations for the authentication of seafood by means of DNA analysis.