Maternal high salt diet altered Adenosine‐mediated vasodilatation via PKA/BK channel pathway in offspring rats. Issue 8 (3rd March 2017)

• Record Type: Journal Article
• Authors: Wu, Jue; Li, Na; Liu, Yanping; Li, Weisheng; He, Axin; Zhu, Di; Feng, Xueqin; Liu, Bailin; Shi, Ruixiu; Zhang, Yujuan; Lv, Juanxiu; Xu, Zhice
• Is Part Of: Molecular nutrition & food research. Volume 61:Issue 8(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-03-03
• Subjects: Adenosine -- Coronary arteries -- Large‐conductance Ca2+‐activated K+ channel -- Prenatal high salt -- Vasodilatation; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201600963
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Adenosine mediates vasodilatation mostly via A2A adenosine receptor, A2A receptors are coupled to GS proteins and activate adenylate cyclase, leading to cyclic Adenosine 5′‐monophosphate (cAMP) accumulation and subsequent activation of protein kinase A (PKA). The activation of K + channels by PKA leads to the dilatation of vessels. The present study demonstrates that prenatal high‐salt diet changes adenosine‐induced vascular dilation in offspring coronary arteries, which is probably associated with the change of cAMP/PKA/K + channel signaling pathway. Abstract : Scope: High salt (HS) diets are related to cardiovascular diseases, and prenatal HS was suggested to increase risks of coronary artery diseases in the offspring. This study tested the hypothesis that prenatal HS may influence Adenosine‐induced vasodilatation via protein kinase A (PKA) pathway in coronary arteries. Methods and results: Sprague–Dawley rats were fed with 8% salt diet for gestation, the control was fed with 0.3% salt diet. Coronary arteries from male adult offspring were tested for K + channels and Adenosine signal pathways. Adenosine‐mediated vasodilatation was reduced in coronary arteries in HS. There was no difference in gene expression of A2A receptors between the two groups. After pretreatment with PKA inhibitor, vasodilatation to Adenosine was decreased to a smaller extent in HS than that in control. Forskolin (activator of adenylate cyclase)‐mediated vasodilatation was decreased in HS. Iberiotoxin (large‐conductance Ca 2+ ‐activated K + channel [BK channel] inhibitor) attenuated Forskolin‐induced vasodilatation in control, not in HS group. Currents of BK channels decreased in coronary artery smooth muscle cells, and PKA‐modulated BK channel functions were declined. Protein levels of BK β1 and PKA C‐subunits in coronary arteries of HS offspring were reduced. Conclusions: Prenatal HS diets altered Adenosine‐mediated coronary artery vasodilatation in the offspring, which was linked to downregulation of cAMP/PKA/BK channel pathway.