Preparation, characterization and evaluation of an inclusion complex of steviolbioside with γ-cyclodextrin. (December 2018)

• Record Type: Journal Article
• Authors: Wan, Hui-da; Ni, Yao; Li, Dan
• Is Part Of: Food bioscience. Volume 26(2018)
• Page Start: 65
• Page End: 72
• Publication Date: 2018-12
• Subjects: Inclusion complex -- Steviol glycosides -- Steviolbioside -- Cyclodextrin; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2018.09.007
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: The preparation, characterization and evaluation of γ -cyclodextrin/steviolbioside ( γ -CD/Sbio) inclusion complex was evaluated. Sbio is a typical steviol glycoside, which is used as a sucrose substitute and has health benefits. To overcome Sbio's poor aqueous solubility and low bioavailability, γ -CD/Sbio was prepared using a co-evaporation method. The possible bonding mode of Sbio with α -CD, β -CD and γ -CD was identified using AutoDock software. Host molecular screening tests showed that γ -CD was the optimal host molecule. The solubility of Sbio increased 31 fold, with a 1:1 γ -CD/Sbio inclusion complex. HPLC, FT-IR, TGA, XRD, SEM and NMR were used to characterize the γ -CD/Sbio. Based on an in vitro test, γ -CD/Sbio was more stable than Sbio with cecal contents. In addition, in vivo pharmacokinetics were done. The Cmax and AUC of γ -CD/Sbio were 2.4 times higher than for Sbio. Overall, the solubility and oral bioavailability of Sbio was significantly improved after inclusion. Graphical abstract: Highlights: γ -CD/Sbio inclusion complexes can be readily prepared using a coevaporation method. The solubility of Sbio can be increased 31 fold after inclusion. The relative oral bioavailability of Sbio was increased by about 2.4 times after inclusion.