Non-competitive immunoassay for low-molecular-weight contaminant detection in food, feed and agricultural products: A mini-review. (January 2018)

• Record Type: Journal Article
• Authors: Liu, Aiping; Anfossi, Laura; Shen, Li; Li, Cheng; Wang, Xiaohong
• Is Part Of: Trends in food science & technology. Volume 71(2018)
• Page Start: 181
• Page End: 187
• Publication Date: 2018-01
• Subjects: Anti-metatype antibody -- Hapten -- Immunosensor -- Non-competitive immunoassay -- Immunocomplex antibody -- Open sandwich immunoassay; Food industry and trade -- Periodicals; Food -- Biotechnology -- Periodicals; 664.005
• DOI: 10.1016/j.tifs.2017.11.014
• Languages: English
• ISSNs: 0924-2244

Abstract

Abstract: Background: Immunoassays have gained considerable attention in safety assurance for food, feed and agricultural products. Generally, immunoassays are presented either in a competitive or non-competitive, sandwich-type format, and the former is extensively employed for low-molecular-weight contaminants, which usually bear one accessible epitope. Theoretically, non-competitive, sandwich-type immunoassays have higher sensitivity, precision and linearity. However, the analyte to be measured in such a format must be large enough to have at least two epitopes to be captured. It is not feasible to detect low-molecular-weight contaminants through conventional non-competitive sandwich-type immunoassay. Consequently, there is a trend to develop new types of sensitive non-competitive immunoassays for low-molecular-weight contaminants. Scope and approach: This article reviews the progress in non-competitive immunoassays for low molecular weight contaminants in food, feed and agricultural products, including the principles, applications and suggested perspectives for this field. Key findings and conclusions: Anti -metatype antibody-based immunoassays are the most promising method, but dissociation of the antibody-hapten complex might be a challenge, and therefore more in-depth research should be focused on preparation of new formats of the antibody-hapten complex. Meanwhile, strategies for direct non-competitive detection or aimed at the simultaneous detection of different targets would be especially desirable besides focusing on improving the sensitivity and specificity of the detection. Highlights: Non-competitive immunoassays are favored due to sensitivity, specificity and linearity. Use of non-competitive immunoassays to detect small molecules in Agri-food products. Future trends to detect low-molecular-weight hazards by non-competitive immunoassays.