Fast and simple determination of moroxydine residues in pig and chicken samples by ultra-performance liquid chromatography-tandem mass spectrometry. Issue 11 (2nd November 2018)

• Record Type: Journal Article
• Authors: Li, Jian; Ming, Meiting; Huai, Wenhui; Cai, Zhimin; Sun, Zhiwen; Ye, Nengsheng
• Is Part Of: Food additives & contaminants. Volume 35:Issue 11(2018)
• Page Start: 2111
• Page End: 2119
• Publication Date: 2018-11-02
• Subjects: Moroxydine -- ultra-performance liquid chromatography-tandem mass spectrometry -- solid-phase extraction -- pig and chicken; Food additives -- Periodicals; Food contamination -- Periodicals; 664.06
• DOI: 10.1080/19440049.2018.1512756
• Languages: English
• ISSNs: 1944-0049

Abstract

ABSTRACT: A general solid-phase extraction (SPE) method using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the determination of moroxydine residues in pig and chicken samples has been developed. After extraction and purification of real samples, moroxydine residues were detected using a hydrophobic interaction liquid chromatography column with an optimised mobile phase composition. The extraction reagents, the kind of SPE columns and the type of eluents were optimised to achieve the maximum extraction efficiency. The matrix effects from the animal tissue influenced the quality of the quantitative data obtained. Under the optimised conditions, the moroxydine residues in pig and chicken samples spiked at three levels (1.0 μg/kg, 5.0 μg/kg and 10.0 μg/kg) were determined with good recoveries (61.5%–105.4%) and adequate relative standard deviations (3.2%–13.0%). In pig and chicken samples, the limit of detection (LOD) was 0.3 μg/kg, and the limit of quantification (LOQ) was 1.0 μg/kg. A sufficiently linear relationship in the range of 1.0 μg/kg–20.0 μg/kg was achieved with a good correlation coefficient (R 2 ≥ 0.99). Graphical Abstract: