SPOP, ZEB-1 and E-cadherin expression in clear cell renal cell carcinoma (cc-RCC): Clinicopathological and prognostic significance. Issue 4 (December 2018)

• Record Type: Journal Article
• Authors: Harb, Ola A.; Elfeky, Mariem A.; El Shafaay, Basant Sh; Taha, Heba F.; Osman, Gamal; Harera, Ibtsam Shehta; Gertallah, Loay M.; Abdelmonem, Doaa Metwaly; Embaby, Ahmed
• Is Part Of: Pathophysiology. Volume 25:Issue 4(2018)
• Page Start: 335
• Page End: 345
• Publication Date: 2018-12
• Subjects: cc-RCC -- SPOP -- ZEB1 -- E-cadherin -- Immunohistochemistry -- Prognosis; Physiology, Pathological -- Periodicals; 571.9
• DOI: 10.1016/j.pathophys.2018.05.004
• Languages: English
• ISSNs: 0928-4680

Abstract

Abstract: Background: Clear cell renal cell carcinoma (cc-RCC), is a serious cancer regarding; its fatality, liability for metastases and chemoresistance, so identification of recent therapeutic targets to improve the patients prognosis is needed. SPOP is a BTB/POZ domain containing speckle-type POZ protein, has been identified as an E3 ubiquitin ligase component. ZEB1 is an essential epithelial mesenchymal transition (EMT) activator; E-cadherin is a cell adhesion protein that had been detected in normal epithelial cells membrane. Aim: Was to assess the tissue protein markers SPOP, ZEB1 & E-cadherin expressions in benign areas of neoplastic kidney specimens and in cc-RCC patients, then correlating their expression levels with patients clinicopathological and prognostic data. Methods: We evaluated SPOP, ZEB-1 & E-cadherin expression using immunohistochemistry in samples from 50 cc-RCC and 20 benign areas of neoplastic kidney specimens, then we followed our patients for 5 years and finally we have analyzed correlations between the levels of markers expressions with patients clinicopathological and prognostic criteria in cc-RCC. Results: Positive expression of SPOP & ZEB1 in addition to negative E- cadherin expression was detected in cc-RCC more than benign areas of neoplastic kidney specimens (p = 0.004 and p < 0.001 respectively). In cc-RCC Positive expression of SPOP, ZEB1 and negative E- cadherin expression was associated with higher grade (p = 0.006, 0.007 & <0.001 respectively), advanced AJCC stage (p = 0.013, 0.023 & <0.001 respectively), presence of L.N metastases (p = 0.002 = 0.010 and <0.001 respectively), distant metastases (p = 0.001, 0.003 & 0.035 respectively), poor PFS and OS rates (p < 0.001 and p = 0.013 respectively). Conclusion: Positive expression of SPOP& ZEB1 in addition to negative E- cadherin are associated with poor prognosis in cc-RCC patients.