Multi-mycotoxin stable isotope dilution LC–MS/MS method for Fusarium toxins in beer. (1st March 2017)

• Record Type: Journal Article
• Authors: Habler, Katharina; Gotthardt, Marina; Schüler, Jan; Rychlik, Michael
• Is Part Of: Food chemistry. Volume 218(2017)
• Page Start: 447
• Page End: 454
• Publication Date: 2017-03-01
• Subjects: Fusarium mycotoxins -- Stable isotope dilution assay -- LC–MS/MS -- Solid phase extraction -- Beer; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.09.100
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Multi-mycotoxin stable isotope dilution LC–MS/MS method established for beer samples. Simultaneous solid phase extraction for trichothecenes, ENNS, BEA and ZEA in beer. In 2015 mycotoxin contamination of beer seems not to be a health risk for consumers. Possibility to follow the fate of Fusarium toxins during the entire brewing process. Abstract: A stable isotope dilution LC–MS/MS multi-mycotoxin method was developed for 12 different Fusarium toxins including modified mycotoxins in beer (deoxynivalenol-3-glucoside, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyl-deoxynivalenol, HT2-toxin, T2-toxin, enniatin B, B1, A1, A, beauvericin and zearalenone). As sample preparation and purification of beer a combined solid phase extraction for trichothecenes, enniatins, beauvericin and zearalenone was firstly developed. The validation of the new method gave satisfying results: intra-day and inter-day precision and recoveries were 1–5%, 2–8% and 72–117%, respectively. In total, 61 different organic and conventional beer samples from Germany and all over the world were analyzed by using the newly developed multi-mycotoxin method. In summary, deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyldeoxynivaleneol and enniatin B were quantified in rather low contents in the investigated beer samples. None of the other monitored Fusarium toxins like 15-acetyldeoxynivalenol, HT2- and T2-toxin, zearalenone, enniatin B1, A1, A or beauvericin were detectable.