Screening of antibiotic residues in pork meat in Ho Chi Minh City, Vietnam, using a microbiological test kit and liquid chromatography/tandem mass spectrometry. (November 2016)

• Record Type: Journal Article
• Authors: Ngoc Do, Mai Hoang; Yamaguchi, Takahiro; Okihashi, Masahiro; Harada, Kazuo; Konishi, Yoshimasa; Uchida, Kotaro; Bui, Long Thi; Nguyen, Thinh Duc; Phan, Ha Bich; Bui, Huong Dang Thien; Nguyen, Phuc Do; Kajimura, Keiji; Kumeda, Yuko; Dang, Chinh Van; Hirata, Kazumasa; Yamamoto, Yoshimasa
• Is Part Of: Food control. Volume 69(2016:Nov.)
• Page Start: 262
• Page End: 266
• Publication Date: 2016-11
• Subjects: Residual antibiotics -- Vietnam -- On-site screening -- Liquid chromatography-tandem mass spectrometry; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2016.05.004
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: The screening and monitoring of residual antibiotics in pork meat were conducted in Ho Chi Minh City, Vietnam, from 2014 to 2015. One hundred samples collected from a wholesale market were screened on-site using a microbiological kit, and eighteen samples were determined as positive for residual antibiotics. Once transferred to the lab, all the samples were analyzed for 28 antibiotics using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Sulfamethazine residues (11–1600 μg/kg) were detected in 23 samples by LC-MS/MS. Notably, 11 samples with sulfamethazine concentrations ranging from 310 to 1600 μg/kg were detected as "positive" in the initial screening, whereas 12 other samples with sulfamethazine levels below 200 μg/kg were screened as "negative". No other targeted antibiotics were identified among the remaining positive samples. These results show the potential and limitations of a monitoring system combining on-site microbiological tests and an LC-MS/MS method. Highlights: Residual antibiotics in pork samples were tested using a microbiological test kit. Sulfamethazine residues were detected in 23 samples by LC-MS/MS analysis in the lab. Eleven showed "positive" in the screening with concentrations of >310 μg/kg. Twelve showed "negative" in the screening with concentrations of <200 μg/kg.