Development of a method for the quantification of fish major allergen parvalbumin in food matrix via liquid chromatography-tandem mass spectrometry with multiple reaction monitoring. (15th March 2019)

• Record Type: Journal Article
• Authors: Sun, Lirui; Lin, Hong; Li, Zhenxing; Sun, Weihong; Wang, Jianhua; Wu, Haiyan; Ge, Minmin; Ahmed, Ishfaq; Pavase, Tushar Ramesh
• Is Part Of: Food chemistry. Volume 276(2019)
• Page Start: 358
• Page End: 365
• Publication Date: 2019-03-15
• Subjects: PV Parvalbumin -- HPLC-MS High performance liquid chromatography-tandem mass spectrometry -- MRM Multiple reaction monitoring -- VITAL Voluntary Incidental Trace Allergen Labeling -- ELISA Enzyme-linked immunosorbent assay -- RT-qPCR Real-time quantitative polymerase chain reaction -- RSD Relative standard deviation -- LOD Limit of detection -- LOQ Limit of quantification -- BCA Bicinchonininc acid -- CE Collision energy -- DP Declustering potential; Tris (PubChem CID:6503) -- Glycine (PubChem CID:750) -- Dithiothreitol (PubChem CID:446094) -- Iodoacetamide (PubChem CID: 3727) -- Ammonium bicarbonate (PubChem CID:14013) -- Formic acid (PubChem CID:284) -- Acetonitrile (PubChem CID:6342) -- Trypsin (PubChem CID:33613); Allergen -- Parvalbumin -- Liquid chromatography -- Mass spectrometry -- Quantification; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.10.014
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Developed a LC-MS/MS method for quantification of β-parvalbumin in flounder. The parameters of chromatography and tryptic digestion were completely optimized. The method was completely validated according to requirements defined by ICH. This method was successfully applied to detect β-parvalbumin in other food matrices. Abstract: The availability of analytical methods for quantification of allergens is crucial for the correct assessment and labeling of products in order to protect allergic consumers. For this purpose, a simple, sensitive and accurate technique was developed based on liquid chromatography-tandem mass spectrometry and multiple reaction monitoring mass spectrometry (LC-MRM-MS/MS). The proposed method uses a simple purification with heat and a completely optimized tryptic digestion. This method has been validated according to the requirements defined by ICH (Q2 [R1]), having a linear range from 0.10 to 1179.36 nM with r > 0.999. The parvalbumin beta in flounder ( Paralichthys olivaceus ) has been quantified at a low level down to 0.10 µg/g with satisfactory precision (RSD < 18.35%) and accuracy (<13.3%). The new approach was successfully applied for the determination of parvalbumin beta in the other food matrices.