Upregulation of an Artificial Zymogen by Proteolysis. Issue 38 (16th August 2016)

• Record Type: Journal Article
• Authors: Liu, Zhe; Lebrun, Vincent; Kitanosono, Taku; Mallin, Hendrik; Köhler, Valentin; Häussinger, Daniel; Hilvert, Donald; Kobayashi, Shu; Ward, Thomas R.
• Is Part Of: Angewandte Chemie international edition. Volume 55:Issue 38(2016)
• Page Start: 11587
• Page End: 11590
• Publication Date: 2016-08-16
• Subjects: artificial metalloenzyme -- artificial zymogen -- asymmetric catalysis -- transfer hydrogenation -- upregulation; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201605010
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Regulation of enzymatic activity is vital to living organisms. Here, we report the development and the genetic optimization of an artificial zymogen requiring the action of a natural protease to upregulate its latent asymmetric transfer hydrogenase activity. Abstract : Cut it on ! An artificial zymogen with latent asymmetric transfer hydrogenase activity was developed and genetically optimized. Its upregulation by a natural protease is demonstrated (see picture; Sav=streptavidin).