Virulence characterization of non-O157 Shiga toxin-producing Escherichia coli isolates from food, humans and animals. (September 2015)

• Record Type: Journal Article
• Authors: Shen, Jinling; Rump, Lydia; Ju, Wenting; Shao, Jingdong; Zhao, Shaohua; Brown, Eric; Meng, Jianghong
• Is Part Of: Food microbiology. Volume 50(2015:Sep.)
• Page Start: 20
• Page End: 27
• Publication Date: 2015-09
• Subjects: STEC -- Shiga toxin subtype -- eae subtype -- Vero cell cytotoxicity; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2015.02.007
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: A total of 359 non-O157 STEC isolates from food, humans and animals were examined for serotypes, Shiga toxin subtypes and intimin subtypes. Isolates solely harboring stx 2 from the three sources were selected for Vero cell cytotoxicity test. stx subtypes in eae negative isolates were more diverse than in eae positive isolates primarily carrying stx 2a . Four eae subtypes ( eae β, eae ε1, eae γ1 and eae γ2/θ ) were observed and correlated with serotypes and flagella. Food isolates showed more diverse serotypes, virulence factors and cell cytotoxicities than human isolates. Some isolates from produce belonged to serotypes that have been implicated in human diseases, carried stx 2a or/and stx 2dact and exhibited high cell cytotoxicity similar to human isolates. This indicates that foods can be contaminated with potentially pathogenic STEC isolates that may cause human diseases. Given the increased produce consumption and growing burden of foodborne outbreaks due to produce, produce safety should be given great importance. Highlights: We analyze serotypes and virulence factors of non-O157 STEC from different sources. We examine vero cell cytotoxicity of stx 2 carrying STEC isolates. stx subtypes in eae negative isolates are more diverse than in eae positive isolates. eae subtypes are correlated with serotypes and flagella. Food isolates show more diverse serotypes and virulence factors than human isolates.