Tomato cystine-knot miniproteins possessing anti-angiogenic activity exhibit in vitro gastrointestinal stability, intestinal absorption and resistance to food industrial processing. (15th April 2017)

• Record Type: Journal Article
• Authors: Treggiari, Davide; Zoccatelli, Gianni; Chignola, Roberto; Molesini, Barbara; Minuz, Pietro; Pandolfini, Tiziana
• Is Part Of: Food chemistry. Volume 221(2017)
• Page Start: 1346
• Page End: 1353
• Publication Date: 2017-04-15
• Subjects: Cystine-knot miniproteins -- Endothelial cell migration -- Gastrointestinal digestion -- Intestinal transport -- Tomato paste; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.11.020
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Tomato cysteine-rich miniproteins (TCMPs) possess anti-angiogenic properties. TCMPs are resistant to gastrointestinal peptidases and actively cross the intestinal mucosa. The industrial processing does not modified TCMPs' biological activities. Abstract: The cystine-knot miniproteins present in tomato fruit (TCMPs) have been shown to exert anti-angiogenic effects by inhibiting endothelial cell migration and to display resistance to gastrointestinal proteolytic attack. To better define the pharmacological potential of TCMPs, their oral bioavailability and their resistance to industrial processing must be assessed. To explore the intestinal transport of TCMPs we used the differentiated Caco-2 cells model. After 24 h incubation, 37.73 ± 9.34% of TCMPs crossed the epithelium, without altering the integrity of the cell layer. To assess the effects of the industrial processing on the biochemical features and the biological activity of TCMPs, we developed a method for purifying the proteins from tomato paste. The tomato-paste purified TCMPs retained the resistance to gastrointestinal digestion and the inhibitory activity towards endothelial cell migration. Our previous and present results collectively demonstrate that TCMPs possess interesting features for drug development.