Effects of high hydrostatic pressure, ultraviolet light-C, and far-infrared treatments on the digestibility, antioxidant and antihypertensive activity of α-casein. (15th April 2017)

• Record Type: Journal Article
• Authors: Hu, Guanlan; Zheng, Yuanrong; Liu, Zhenmin; Xiao, Yang; Deng, Yun; Zhao, Yanyun
• Is Part Of: Food chemistry. Volume 221(2017)
• Page Start: 1860
• Page End: 1866
• Publication Date: 2017-04-15
• Subjects: CN casein -- α-CN α-casein -- HPP high hydrostatic pressure -- UV-C ultraviolet light-c -- FIR far-infrared radiation -- ACE angiotensin-converting-enzyme -- DPPH 1, 1-diphenyl-2-picrylhydrazyl -- ABTS 2, 2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) -- RP reducing power; High hydrostatic pressure -- UV-C -- Far-IR -- Antihypertensive -- Antioxidant -- Digestibility -- α-Casein; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.10.088
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: High pressure, UV-C, and far-IR treatments improved the ACE inhibitory activity. One 5-min cycle at 200 MPa led to the highest peptide yields and digestibility. UV-C (15 min) treatment caused the highest DPPH radical-scavenging activity. Abstract: Alpha-casein is the most important bioactive protein in processing technologies. This study investigated the digestibility, antioxidant and antihypertensive activities of α-casein when treated by high hydrostatic pressure (HPP), ultraviolet light-C (UV-C), and far-infrared radiation (FIR). The in vitro digestibility was modified after treatments, especially after 5 min/200 MPa HHP treatment. Ultra-performance liquid chromatography-tandem mass spectrometry analysis showed that one 5 min/200 MPa HHP treatment resulted in the highest yield of peptides. Based on the in vitro gastrointestinal digestion and antioxidant and antihypertensive activity assays, HHP increased the angiotensin converting enzyme inhibitory activity at different levels. The 15 min UV-C treatment resulted in the highest antioxidant DPPH radical-scavenging activity, while the 15 min UV-C and FIR treatments had higher angiotensin converting enzyme inhibitory and antioxidant activities than those of 5 min treatments. This study revealed that HHP, UV-C and FIR treatments increased the antioxidant and antihypertensive activities of α-casein.