Impairment of hormone pathways results in a general disturbance of fruit primary metabolism in tomato. (15th February 2019)

• Record Type: Journal Article
• Authors: Li, Ying; Lu, Yongen; Li, Lili; Chu, Zhuannan; Zhang, Hongyan; Li, Hanxia; Fernie, Alisdair R.; Ouyang, Bo
• Is Part Of: Food chemistry. Volume 274(2019)
• Page Start: 170
• Page End: 179
• Publication Date: 2019-02-15
• Subjects: Sucrose (PubChem CID: 5988) -- Glucose (PubChem CID: 5793) -- Fructose (PubChem CID: 5984) -- Malic acid (PubChem CID: 525) -- Citric acid (PubChem CID: 311) -- Glutamic acid (PubChem CID: 611) -- Glutamine (PubChem CID: 5961) -- Phenylalanine (PubChem CID: 6140); GC–MS -- Metabolome -- Micro-Tom -- Hormone mutants -- PCA -- Fruit stage; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.08.026
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Primary metabolism altered globally in five tomato hormone mutants. ABA has the largest impact on primary metabolism. Ethylene seems important in the transition of primary to secondary metabolism. Abstract: Fruit metabolites are regulated by different phytohormones; however, this needs to be investigated. Dynamic metabolite profiling, based on gas chromatography-mass spectrometry, has been conducted on the fruit of tomato cultivar Micro-Tom and its five hormone mutants: dpy, not, dgt, epi and pro . In total, 48 metabolites were quantified, including sugars, organic acids and amino acids. The results demonstrated that ABA had a greater effect on the regulation of primary metabolism in tomato fruit, while ethylene can play an important role in the transition of primary to secondary metabolism. Besides, results from enzyme activities and transcript abundance involved in primary metabolism suggested that AIV and HXK4 could play key roles in the accumulation of the main sugars. To the best of our knowledge, this is the first comprehensive analysis of the link between hormone and metabolite change during fruit development in a collection of mutants with diverse hormone pathways.