Antioxidant effects of gastrointestinal digested purple carrot extract on the human cells of colonic mucosa. (1st January 2016)

• Record Type: Journal Article
• Authors: Olejnik, Anna; Rychlik, Joanna; Kidoń, Marcin; Czapski, Janusz; Kowalska, Katarzyna; Juzwa, Wojciech; Olkowicz, Mariola; Dembczyński, Radosław; Moyer, Mary Pat
• Is Part Of: Food chemistry. Volume 190(2016)
• Page Start: 1069
• Page End: 1077
• Publication Date: 2016-01-01
• Subjects: Purple carrot -- Anthocyanins -- Antioxidant capacity -- In vitro digestion -- Colon mucosa -- Oxidative stress -- DNA damage -- Intracellular ROS; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.06.080
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Loss of the purple carrot anthocyanins under gastrointestinal digestion. Purple carrot extract reduces excessive ROS production in colon cells. Purple carrot extract decreases oxidative DNA damage in colon cells. Non-digested purple carrot extract is more potent than colon digested extract. Low cytotoxicity of purple carrot extract to normal colon cells. Abstract: Purple carrot (PC) is a potential dietary constituent, which represents a valuable source of antioxidants and can modulate the reactive oxygen species (ROS) level in the gastrointestinal tract. Antioxidant capacity of a PC extract subjected to digestion process simulated in the artificial alimentary tract, including the stomach, small intestine and colon, was analyzed in normal human cells of colon mucosa. Results indicated that the extract obtained upon passage through the gastrointestinal tract, which could come into contact with the colonic cells in situ, was less potent than the extract, which was not subjected to digestion process. Digested PC extract exhibited intracellular ROS-inhibitory capacity, with 1 mg/mL showing the ROS clearance of 18.4%. A 20.7% reduction in oxidative DNA damage due to colon mucosa cells' treatment with digested PC extract was observed. These findings indicate that PC extract is capable of colonic cells' protection against the adverse effects of oxidative stress.