Synthesis of biofunctionalized silica nanospheres to separate GST-tagged proteins. Issue 6 (June 2016)

• Record Type: Journal Article
• Authors: Zou, Xueyan; Li, Liangliang; Lu, Haitao; Zhang, Yutao; Zhao, Yanbao; Zhang, Yu; Guo, Quanhui
• Is Part Of: Process biochemistry. Volume 51:Issue 6(2016:Jun.)
• Page Start: 804
• Page End: 808
• Publication Date: 2016-06
• Subjects: Silica hollow nanospheres -- GST-tagged -- Surface functionalization -- Purification; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2016.03.010
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Hollow silica nanospheres (NSs) with Glutatione group (SiO2 -GSH) have been prepared. SiO2 -GSH NSs were applied to purify GST-tagged proteins without any preprocessing. The capacity of separated protein barely changed after three times reuse. The detection limit is lower than 1.0 × 10 −6 mol/L to the target proteins. The separated capacity of the SiO2 -GSH NSs is as much as 89.9 μmol/g. Abstract: A Thiol-functionalized silica hollow nanospheres(denoted as SiO2 -SH NSs) were prepared through a hydrothermal route. The SiO2 -SH NSs were conjugated with glutathione group (denoted asGSH) to afford SiO2 -GSH NSs. The as-prepared SiO2 -GSH sample has hollow structure and exhibits an average diameter of about 45 nm and a wall thickness of 10 nm. The SiO2 -GSH NSs were used to separate three kinds of GST-tagged proteins (GST-tagged GPX, GST-tagged LOV and GST-tagged 210-6P). These SiO2 -GSH NSs exhibit negligible non-specific adsorption, high binding capacity (89.9 μmol/g), the low detection limit (1.0 × 10 −6 mol/L) and reuse property, showing great potentiality in purifying GST-tagged proteins.