Chemical composition and "in vitro" anti-inflammatory activity of Vitis vinifera L. (var. Sangiovese) tendrils extract. (January 2016)

• Record Type: Journal Article
• Authors: Fraternale, Daniele; Rudov, Alexander; Prattichizzo, Francesco; Olivieri, Fabiola; Ricci, Donata; Giacomini, Elisa; Carloni, Silvia; Azzolini, Catia; Gordillo, Belén; Jara-Palacios, M. José; Verardo, Giancarlo; Albertini, Maria Cristina
• Is Part Of: Journal of functional foods. Volume 20(2016)
• Page Start: 291
• Page End: 302
• Publication Date: 2016-01
• Subjects: CAD coronary artery disease -- CVD cardiovascular disease -- DPPH diphenylpicrylhydrazyl -- HPLC-DAD high-performance liquid chromatography with diode-array detection -- IRAK-1 IL-1 receptor-associated kinase 1 -- LPS lipopolysaccharide -- MIRAKIL biomarkers associated to SASP (miR-146a expression, IRAK-1, IL-6 released into the culture medium) -- NCTC 2544 human keratinocytes -- P13 endothelial cells cultured until the 13th passage -- PAI-1 plasminogen activator inhibitor type 1 -- PBMCs peripheral blood mononuclear cells -- PMA phorbol 12-myristate 13-acetate -- SASP senescence-associated secretory phenotype -- T2DM type 2 diabetes mellitus -- TRAF6 TNF receptor-associated factor 6 -- TVV grape tendril extract; Vitis vinifera tendrils -- Anti-inflammatory activity -- miR-126 -- miR-146a -- MIRAKIL; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.11.013
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Grapevine tendril extract (TVV) contains anti-oxidant/anti-inflammatory compounds. TVV showed anti-oxidant properties on U937 H2 O2 treated cells. TVV showed anti-inflammatory effect on U937 and HUVEC LPS stimulated cells. TLR4 signalling is modulated by TVV treatment. TVV may prevent inflammatory associated diseases. Abstract: Grapevine ( Vitis vinifera L.) is currently used for wine production, herbal medicine and food supplements. In this study we investigated the phenolic composition of grape tendril extract (TVV), because these compounds are probably responsible for the observed biological effects of TVV. We performed our investigation on cells implicated in the inflammatory process and in vascular damage, such as monocyte (U937) and senescent endothelial (HUVEC) cells. We analysed changes in microRNA (miRNA) expression during a pro-inflammatory condition in LPS (lipopolysaccharide) stimulated cells. MiR-126, defined as angiomiR for its ability in the maintenance of endothelial cell functions, and miR-146a, defined as inflamma-miR, for its ability to down-modulate (NF-kB nuclear factor-kB) pro-inflammatory pathway, were analysed along with their targets. TVV extract (40 µg/mL) was able to prevent the LPS stimulus and may play an important role in the prevention of endothelial dysfunction when the accumulation of senescent endothelial cells occurs.