Identification of specific bovine blood biomarkers with a non-targeted approach using HPLC ESI tandem mass spectrometry. (15th December 2016)

• Record Type: Journal Article
• Authors: Lecrenier, M.C.; Marbaix, H.; Dieu, M.; Veys, P.; Saegerman, C.; Raes, M.; Baeten, V.
• Is Part Of: Food chemistry. Volume 213(2016)
• Page Start: 417
• Page End: 424
• Publication Date: 2016-12-15
• Subjects: Processed animal proteins (PAPs) -- Bovine spongiform encephalopathy (BSE) -- Bovine -- Blood -- Biomarkers -- Tandem mass spectrometry (MS/MS); Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.06.113
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Selection of the proteins of interest using an analytical approach. Identification of specific bovine and porcine biomarkers to detect processed blood. Two biomarker types: for plasma powder or for haemoglobin powder and blood meal. Evaluation of the specificity against commercial feeds and feed materials. Biomarkers could be used to ensure the implementation of regulations in feed safety. Abstract: Animal by-products are valuable protein sources in animal nutrition. Among them are blood products and blood meal, which are used as high-quality material for their beneficial effects on growth and health. Within the framework of the feed ban relaxation, the development of complementary methods in order to refine the identification of processed animal proteins remains challenging. The aim of this study was to identify specific biomarkers that would allow the detection of bovine blood products and processed animal proteins using tandem mass spectrometry. Seventeen biomarkers were identified: nine peptides for bovine plasma powder; seven peptides for bovine haemoglobin powder, including six peptides for bovine blood meal; and one peptide for porcine blood. They were not detected in several commercial compound feed or feed materials, such as blood by-products of other animal origins, milk-derived products and fish meal. These biomarkers could be used for developing a species-specific and blood-specific detection method.