Quadrupole time-of-flight mass spectrometry analysis of glycerophospholipid molecular species in the two halophyte seed oils: Eryngium maritimum and Cakile maritima. (15th December 2016)

• Record Type: Journal Article
• Authors: Zitouni, Manel; Wewer, Vera; Dörmann, Peter; Abdelly, Chedly; Ben Youssef, Nabil
• Is Part Of: Food chemistry. Volume 213(2016)
• Page Start: 319
• Page End: 328
• Publication Date: 2016-12-15
• Subjects: PLs phospholipids -- GPLs glycerophospholipids -- PC phosphatidylcholine -- PE phosphatidylethanolamine -- PI phosphatidylinositol -- PA phosphatidic acid -- PG phosphatidylglycerol -- PS phosphatidylserine -- Q-TOF quadrupole time of flight -- MS mass spectrometry -- E. maritimum Eryngium maritimum -- C. maritima Cakile maritima -- CID collision induced dissociation -- EIC extracted ion chromatogram -- Q-TOF-MS Quadrupole time-of-flight mass spectrometry -- MS–MS tandem mass spectrometry -- FW fresh weight -- FAMS fatty acid methyl esters -- C16:0 palmitic acid -- C18:0 stearic acid -- C18:1 oleic acid -- C18:2 linoleic acid -- C18:3 alpha-linolenic acid -- C22:1 erucic acid -- UFAs unsaturated fatty acids -- SFAs saturated fatty acids; Palmitic acid (pubChem CID: 985) -- Palmitoleic acid (pubChem CID: 445638) -- Stearic acid (pubChem CID: 5281) -- Oleic acid (pubChem CID: 445639) -- Linoleic acid (pub Chem CID: 5280450) -- Alpha-linolenic acid (pubChem CID: 5280934) -- Arachidic acid (pub Chem CID: 10467) -- Gondoic acid (pubChem CID: 5282768) -- Eicosadienoic acid (pubChem CID: 6439848) -- Erucic acid (pubChem CID: 5281116); Eryngium maritimum -- Cakile maritima -- Fatty acids -- Glycerophospholipids -- Molecular species -- Q-TOF MS/MS; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.06.083
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Valorization of two Tunisian halophytic plants in terms of glycerophospholipids. Quantification of about 300 molecular species by QTOF-MS-MS for the first time. Molecular species composition varies quantitatively among the two halophytes. Eryngium maritimum could be considered as a good source of glycerophospholipids. Abstract: Future applications of lipids in clinical cohort studies demand detailed glycerophospholipid molecule information and the application of high-throughput lipidomics platforms. In the present work, a novel sensitive technique with high mass resolution and accuracy was applied to accomplish phospholipid analysis. Nanospray ionization quadrupole time-of-flight mass spectrometry was used to separate and quantify the glycerophospholipid classes as well as molecular species in two halophyte seed oils from Cakile maritima and Eryngium maritimum . Precursor or neutral loss scans of their polar head groups allowed the detection of molecular species within particular glycerophospholipid classes. Phosphatidylcholine was found to be the most abundant glycerophospholipid in both seed oils whereas phosphatidylethanolamine and phosphatidic acid were less abundant. Phosphatidylinositol, phosphatidylserine and phosphatidylglycerol were minor glycerophospholipids. Several molecular species within each class were detected and the main molecular species (C36:4, C36:3, C36:2, 34:2 and C34:1) were quantitatively different between the two halophytes and the different glycerophospholipids.