Purification and identification of an angiotensin I-converting enzyme inhibitory peptide from cauliflower by-products protein hydrolysate. Issue 9 (September 2016)

• Record Type: Journal Article
• Authors: Xu, Yang; Bao, Tao; Han, Wen; Chen, Wei; Zheng, Xiaodong; Wang, Jianxu
• Is Part Of: Process biochemistry. Volume 51:Issue 9(2016:Sep.)
• Page Start: 1299
• Page End: 1305
• Publication Date: 2016-09
• Subjects: Cauliflower by-products protein -- Enzymatic hydrolysis -- ACE inhibitory peptide -- Purification and identification; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2016.05.023
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Alcalase hydrolysate exhibited the highest ACE inhibitory activity. An ACE inhibitory peptide (Val-Trp) was isolated and identified. This peptide had a potent ACE inhibitory activity with an IC50 value of 31.30 μ M. About 42 mg of dipeptide was obtained from 1 kg of fresh Cauliflower by-products. Abstract: Cauliflower by-products (stems and leaves) are rich in leaf protein. Making full use of cauliflower by-products can not only alleviate the shortage of protein resources but also be beneficial to the environmental protection. This study sought to prepare and purify an angiotensin I-converting enzyme (ACE) inhibitory peptide from cauliflower by-products protein hydrolysate. Our result showed that the highest ACE inhibitory activity was observed in the Alcalase 2.4 L hydrolysate among tested hydrolysates (pepsin, pancreatin, and Alcalase 2.4L). After purification, a dipeptide (Val-Trp) was identified, with a molecular weight of 304.1665 Da. This peptide had a potent ACE inhibitory activity with IC50 of 31.30 μ M. Based on this purification process, approximately 42 mg of this dipeptide was obtained from 1 kg of fresh cauliflower by-products. Overall, the results suggest that cauliflower by-products protein can be used as an economical source of functional food material against hypertension.