Comparison of the antiviral activity of flavonoids against murine norovirus and feline calicivirus. (February 2016)

• Record Type: Journal Article
• Authors: Seo, Dong Joo; Jeon, Su Been; Oh, Hyejin; Lee, Bog-Hieu; Lee, Sook-Young; Oh, Seung Hyun; Jung, Ji Youn; Choi, Changsun
• Is Part Of: Food control. Volume 60(2016:Feb.)
• Page Start: 25
• Page End: 30
• Publication Date: 2016-02
• Subjects: Norovirus -- Flavonoid -- Antiviral -- Structure-activity relationship (SAR) -- Feline calicivirus (FCV); Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2015.07.023
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Antiviral effect of 10 flavonoids against feline calicivirus (FCV) and murine norovirus (MNV) was investigated on Crandell-Reese feline kidney (CRFK) cells and RAW 264.7 cells. Each cells infected with FCV or MNV was treated with different flavonoids at concentrations between 50 and 300 μM. A 200 μM dose of kaempferol and daidzein significantly reduced the FCV titer by 69.76 ± 8.71 and 63.47 ± 9.82%, respectively. A 300 μM dose of quercetin and fisetin reduced the titer of FCV titer by 56.70 ± 11.39 and 41.84 ± 11.35%, respectively. The titer of MNV was reduced by 50.47 ± 6.91 and 51.21 ± 13.38% by a 50 μM dose of fisetin or a 150 μM dose of epicatechin gallate, respectively. A 50 μM dose of daidzein and 100 μM of quercetin reduced the MNV titer by 46.32 ± 8.70 and 45.78 ± 9.81%, respectively. The pre-treatment of quercetin, fisetin, daidzein, and epicatechin gallate demonstrated the anti-noroviral activity against MNV and FCV. Highlights: Pre-treatment of flavonoids significantly reduced norovirus surrogates. Co- and post-treatment of flavonoids had no effect to reduce norovirus surrogates. Quercetin, fisetin, epicatechin 3-gallate, and daizein showed the significant reduction of MNV. Kaempferol, quercetin, fisetin, and daidzein showed the significant reduction of FCV.