Addition of abscisic acid increases the production of chitin deacetylase by Colletotrichum gloeosporioides in submerged culture. Issue 8 (August 2016)

• Record Type: Journal Article
• Authors: Ramos-Puebla, Angelica; De Santiago, Carolina; Trombotto, Stéphane; David, Laurent; Larralde-Corona, Claudia Patricia; Shirai, Keiko
• Is Part Of: Process biochemistry. Volume 51:Issue 8(2016:Aug.)
• Page Start: 959
• Page End: 966
• Publication Date: 2016-08
• Subjects: Tween 80 (PubChem CID: 5281955) -- Glucose (PubChem CID: 5793) -- l-Glutamic acid (PubChem CID: 33032) -- Thiamine hydrochloride (PubChem CID: 6202) -- Nicotinic acid hydrochloride (PubChem CID: 71558) -- N-Acetyl-d-glucosamine (PubChem CID: 439174) -- d-Glucosamine (PubChem CID: 439213) -- Indole-3-acetic acid (PubChem CID: 802) -- Gibberellic acid (PubChem CID: 6466) -- Abscisic acid (PubChem CID: 5280896); Chitin deacetylases -- Colletotrichum gloeosporioides -- Phytohormones -- Fed-batch culture; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2016.05.003
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Abscisic acid (ABA) accelerates germination process of Colletotrichum gloeosporioides and increases chitin deacetylases production. Highlights: Abscisic acid (ABA) exerted a positive and significant effect on enzyme production. ABA accelerates the germination process of C. gloeosporioides and increases CDA production. A significant increase in CDA activity was achieved with fed batch. ABA changes acetylated fractions of chitin from fungal cell walls. Abstract: The activity of chitin deacetylase from Colletotrichum gloeosporioides was studied by the addition of phytohormones (gibberellic acid, indole acetic acid, and abscisic acid) and amino sugars (glucosamine and N -acetyl glucosamine) in culture media. Abscisic acid exerted a positive and significant effect on enzyme production with 9.5-fold higher activity (1.05 U mg protein −1 ) than the control (0.11 U mg protein −1 ). Subsequently, this phytohormone was used in batch culture with higher chitin deacetylase activity being found at acidic pH (3.5) than at neutral pH (7). Furthermore, the highest activity was determined at the acceleration growth phase. The chitin deacetylase production was ascribed to the lag phase within the spore germination process and germ tube elongation instead of during the formation of appressoria, as evidenced by the scanning electronic microscopy results. Therefore, more inoculum and medium containing abscisic acid were added to the fed-batch culture, resulting in a significant increase in chitin deacetylase activity (3.64 U mg protein −1 ). The addition of abscisic acid led to changes in the acetylation degree of chitin extracted from the cell wall of C. gloeosporioides, with lower degree of acetylation (DA of 75.6%) than that determined with the culture without abscisic acid (DA of 90.6%).