Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants. Issue 4 (April 2016)

• Record Type: Journal Article
• Authors: Vicente, Filipa A.; Lario, Luciana D.; Pessoa, Adalberto; Ventura, Sónia P.M.
• Is Part Of: Process biochemistry. Volume 51:Issue 4(2016:Apr.)
• Page Start: 528
• Page End: 534
• Publication Date: 2016-04
• Subjects: Bromelain -- Aqueous micellar two-phase system -- Triton X-114 -- Ionic liquids -- Co-surfactant -- Purification; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2016.01.004
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Bromelain is a set of cysteine proteinases crucial in the pharmaceutical field. Bromelain purification was tested using AMTPS based in ILs as co-surfactants. It is notorious the stabilizing effect of phosphonium-based ILs on the bromelain. High bromelain recovery (90%) for the micelle-poor phase was found for all systems. Abstract: Pineapple processing industries produce a large amount of residues; for example, the pineapple stem, which is rich in bromelain. Bromelain is a group of cysteine proteinases that have major importance in the pharmaceutical field due to, among others, their anti-inflammatory, antithrombotic, and fibrinolytic activities. Since Brazil is one of the largest producers of pineapples in the world, the development of an ecofriendly and cost-effective process to extract and purify bromelain from food/fruit residues would be significant. In this study, aqueous micellar two-phase systems composed of ionic liquids as co-surfactants were evaluated in the extraction of bromelain from the pineapple stem crude extract. The main results showed that bromelain partitions preferentially toward the micelle-poor phase, with enzyme recoveries above 90% for the studied systems. Moreover, a stabilizing effect of the ionic liquids toward the enzyme was observed.