C- and O-glycosyl flavonoids in Sanguinello and Tarocco blood orange (Citrus sinensis (L.) Osbeck) juice: Identification and influence on antioxidant properties and acetylcholinesterase activity. (1st April 2016)

• Record Type: Journal Article
• Authors: Barreca, Davide; Gattuso, Giuseppe; Laganà, Giuseppina; Leuzzi, Ugo; Bellocco, Ersilia
• Is Part Of: Food chemistry. Volume 196(2016)
• Page Start: 619
• Page End: 627
• Publication Date: 2016-04-01
• Subjects: ABTS 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) -- AChE acetylcholinesterase -- DPPH 2, 2-diphenyl-1-picrylhydrazyl -- Trolox 6-hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid; Citrus sinensis (L.) Osbeck -- Sanguinello and Tarocco blood orange -- HPLC–DAD–ESI-MS-MS -- Flavonoids -- Antioxidant activity -- Acetylcholinesterase activity; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.09.098
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Twelve flavonoids were identified/quantified in Sanguinello and Tarocco blood orange. Flavonoids strongly influence the antioxidant activity of Sanguinello and Tarocco. Sanguinello and Tarocco juices display remarkable acetylcholinesterase inhibition. Abstract: Sanguinello and Tarocco are the blood orange ( Citrus sinensis (L.) Osbeck) cultivars most diffused worldwide. Reversed phase liquid chromatography coupled with MS-MS analysis showed that these two varieties have a similar chromatographic pattern, characterised by the presence of C - and O -glycosyl flavonoids. Of the two, Sanguinello was found to be far richer in flavonoids than Tarocco. In the juices, twelve individual components were identified for the first time, namely, four C -glycosyl flavones (lucenin-2, vicenin-2, stellarin-2, lucenin-2 4′-methyl ether and scoparin), three flavonol derivatives (quercetin-3- O -(2-rhamnosyl)-rutinoside, quercetin-3- O -hexoside, quercetin 3-hydroxy-3-methylglutaryl-glycoside), an O -triglycosyl flavanone (narirutin 4′- O -glucoside) and a flavone O -glycosides (chrysoeriol 7- O -neoesperidoside). Moreover, the influence of the identified C - and O -glycosyl flavonoids on the antioxidant and acetylcholinesterase activity of these juices has been evaluated.