Characterization, antioxidant and antitumor activities of polysaccharides from purple sweet potato. (5th November 2015)

• Record Type: Journal Article
• Authors: Wu, Qiongying; Qu, Hongsen; Jia, Junqiang; Kuang, Cong; Wen, Yan; Yan, Hui; Gui, Zhongzheng
• Is Part Of: Carbohydrate polymers. Volume 132(2015)
• Page Start: 31
• Page End: 40
• Publication Date: 2015-11-05
• Subjects: Purple sweet potato -- Polysaccharides -- Antioxidant activity -- Antitumor activity; PSPP purple sweet potato polysaccharide -- DPPH 1, 1-diphenyl-2-picrylhydrazyl -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- DMSO dimethyl sulfoxide -- DMEM dulbecco's modified eagle medium -- FBS fetal bovine serum -- GC gas chromatography -- TFA trifluoroacetic acid -- SEM scanning electron microscopy -- FT-IR Fourier transformation infrared; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.06.045
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Three pure polysaccharides, PSPP1-1, PSPP2-1 and PSPP3-1, were isolated from purple sweet potato. PSPP1-1 and PSPP3-1 were composed of Rha, Xyl, Glc and Gal; PSPP2-1 was composed of Rha and Gal. PSPP2-1 had the highest antioxidant activities on DPPH radical, Fe 2+ and reducing power. PSPP1-1 exhibited the strongest antitumor activities against SGC7901 and SW620 cells. Abstract: Three polysaccharides, PSPP1-1, PSPP2-1 and PSPP3-1, were isolated from purple sweet potato. The three polysaccharides belonged to β-type polysaccharides and contained low proportions of proteins and uronic acids. PSPP1-1 and PSPP3-1 with molecular weights of 33.3 and 75.3 kDa, respectively, were composed of rhamnose, xylose, glucose and galactose, whereas PSPP2-1 with molecular weight of 17.8 kDa was composed of rhamnose and galactose. The three polysaccharides possessed in vitro antioxidant (scavenging DPPH radicals, chelating ferrous ions and reducing power) and antitumor activities (against SGC7901 and SW620 cells) in a dose-dependent manner. Among the three polysaccharides, PSPP2-1 exhibited the strongest reducing power, scavenging activity on DPPH radicals and chelating capability on ferrous ions. PSPP1-1 showed the strongest inhibitory activities on the growth of SGC7901 and SW620 cells. In addition, flow cytometry results showed that PSPP1-1 could induce apoptosis in SGC7901 and SW620 cells. These results suggest that polysaccharides from purple sweet potato are potential natural antioxidant and antitumor agents that can be used as drugs or functional food ingredients.