Different glyceollin synthesis-related metabolic content and gene expressions in soybean callus suspension cultures and cotyledon tissues induced by alginate oligosaccharides. (October 2018)

• Record Type: Journal Article
• Authors: Wang, Kaiqiang; Peng, Qing; Qiao, Yu; Li, Yang; Suo, Decheng; Shi, Bo
• Is Part Of: Process biochemistry. Volume 73(2018)
• Page Start: 188
• Page End: 196
• Publication Date: 2018-10
• Subjects: AOS alginate oligosaccharides -- CHI1 chalcone isomerase 1 -- CHI2 chalcone isomerase 2 -- CS callus suspension cultures -- CYP93A1 3, 9-dihydroxypterocarpan 6a-monooxygenase -- 2, 4-D 2, 4-dichlorophenoxyacetic acid -- ECS elicited callus suspension cultures -- ECT elicited cotyledon tissues -- G2DT glycinol 2-dimethylallyltransferase -- G4DT glycinol 4-dimethylallyltransferase -- GS glyceollin synthase -- 2HID 2-hydroxyisoflavanone dehydratase -- IFS1 2-hydroxyisoflavanone synthase 1 -- IFS2 2-hydroxyisoflavanone synthase 2 -- MS Murashige and Skoog -- MT malonyltransferase -- qRT-PCR quantitative real-time polymerase chain reaction -- UCS unelicited callus suspension cultures -- UCT unelicited cotyledon tissues -- UGT uridine diphosphate glycosyltransferase; Glyceollin -- Elicitation -- Callus suspension cultures -- Cotyledon tissues -- Alginate oligosaccharides -- Glycine max; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2018.08.013
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Glyceollin produced by soybean callus suspension cultures upon AOS was first found. Compound content and gene expression changed during glyceollin synthesis under AOS. Glyceollin I was prominent among isomers in AOS-treated callus suspension cultures. Glyceollin II and III were dominant among isomers in AOS-induced soybean cotyledon tissues. Abstract: Glyceollins are a group of inducible soybean phytoalexins. The aim of the present study was to investigate the mechanism of glyceollin production in callus suspension cultures (CS) and cotyledon tissues elicited with alginate oligosaccharides (AOS) at 1%, 2%, and 3% concentrations that were applied for three days. HPLC analysis of the extracts from 2% AOS-elicited callus suspension cultures (ECS) revealed that glyceollins content was 85.3-fold and 8.2-fold higher than that in unelicited controls and elicited cotyledon tissues (ECT), respectively. UPLC-Q-Exactive Orbitrap MS analysis showed that the 2% AOS induction resulted in elevated levels of metabolites, the intermediary products of glyceollin biosynthesis, such as daidzein, glycitein, genistein, naringenin, glycinol, glyceollidin I/II, and glyceollin I, II, and III in ECS and ECT. Glyceollin I was prominent among the three glyceollin isomers in ECS, whereas isomer II and III were dominant in the synthetic route for glyceollins in ECT. qRT-PCR analysis of related genes showed that UGT, CYP93A1, G2DT, and G4DT displayed a direct effect on glyceollin synthesis. Our results suggested that 2% AOS-induced CS produced glyceollins in higher content compared to ECT by altering metabolite content and gene expression. Elicitation of CS is thus a promising technology for glyceollin synthesis extensively.